Estrutura genética intrapopulacional em Cryptocarya moschata Nees (Lauraceae)
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Data
2004-09-01
Autores
Moraes, Pedro L.R. de
Monteiro, Reinaldo [UNESP]
Vencovsky, Roland
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Botânica de São Paulo
Resumo
Pela análise de seis sistemas enzimáticos (ACP, ALP, CAT, GOT, PPO e PO), com 18 locos polimórficos, foram calculadas as freqüências alélicas de 24 alelos referentes a 141 indivíduos adultos de uma população natural de Cryptocarya moschata (noz-moscada-do-Brasil) do Parque Estadual Carlos Botelho (24º03'21 S, 47º59'36 W), São Miguel Arcanjo, SP, amostrados em uma área de cerca de 647 ha. A estrutura genética espacial foi investigada através do método de autocorrelação espacial, utilizando-se coeficientes I de Moran estimados para 10 classes de distância. Adicionalmente, para as mesmas classes de distância, estimaram-se os coeficientes r através de uma análise multivariada. Ambas as abordagens mostraram-se similares qualitativamente, sendo que 15 correlogramas de coeficientes I foram significativos ao nível de 95% de probabilidade. O correlograma total gerado pelos coeficientes r indicou estruturação espacial significativa para a classe de distância de 0 a 150 m. Pela subdivisão desta classe em intervalos de 10 m, detectou-se autocorrelação espacial positiva na maioria das classes, indicando que os agrupamentos de árvores de C. moschata, que se encontram separadas a distâncias menores que 50 m, são constituídos por indivíduos semelhantes geneticamente e provavelmente aparentados. Contudo, a maior similaridade genética ocorreu entre indivíduos distanciados em torno de 105 m, podendo estar associada à dispersão de sementes promovida por Brachyteles arachnoides (Primates - Cebidae). Pela análise dos resultados, conclui-se que para uma amostragem adequada, visando a detecção de diversidade genética, a coleta de indivíduos distanciados acima de 750 m seria recomendável.
Through the analysis of six enzyme systems (ACP, ALP, CAT, GOT, PPO and PO), with 18 polymorphic loci, allele frequencies of 24 alleles were calculated for 141 adult individuals of a natural population of Cryptocarya moschata (Brazilian nutmeg), from Carlos Botelho State Park (24º03'21 S, 47º59'36 W), São Miguel Arcanjo, SP, Brazil, which were sampled in an area ca. 647 ha. The spatial genetic structure was investigated by the spatial autocorrelation method, using Moran's I coefficients estimated for 10 distance classes. Additionally, for the same distance classes, coefficients r were calculated through a multivariate analysis. Both approaches were qualitatively similar, poiting out that 15 correlograms from Moran's I coefficients were significant at 95% probability level. The total correlogram from r coefficients indicated that there was a significant spatial structure for the distance class of 0 to 150 m. By subdividing this class into 10 m intervals, positive spatial autocorrelation was detected in the majority of classes, suggesting that clusters of C. moschata trees, which were shorter than 50 m apart, were constituted of individuals with greater genetic similarity and were probably related. However, the greatest genetic similarity occurred among individuals being 105 m apart, which could be related to the kind of seed dispersal promoted by Brachyteles arachnoides (Primates - Cebidae). Results showed that for an adequate sampling, aiming to detect genetic diversity, the collection of individuals separated by a distance of 750 m, or more, from each other, would be advisable.
Through the analysis of six enzyme systems (ACP, ALP, CAT, GOT, PPO and PO), with 18 polymorphic loci, allele frequencies of 24 alleles were calculated for 141 adult individuals of a natural population of Cryptocarya moschata (Brazilian nutmeg), from Carlos Botelho State Park (24º03'21 S, 47º59'36 W), São Miguel Arcanjo, SP, Brazil, which were sampled in an area ca. 647 ha. The spatial genetic structure was investigated by the spatial autocorrelation method, using Moran's I coefficients estimated for 10 distance classes. Additionally, for the same distance classes, coefficients r were calculated through a multivariate analysis. Both approaches were qualitatively similar, poiting out that 15 correlograms from Moran's I coefficients were significant at 95% probability level. The total correlogram from r coefficients indicated that there was a significant spatial structure for the distance class of 0 to 150 m. By subdividing this class into 10 m intervals, positive spatial autocorrelation was detected in the majority of classes, suggesting that clusters of C. moschata trees, which were shorter than 50 m apart, were constituted of individuals with greater genetic similarity and were probably related. However, the greatest genetic similarity occurred among individuals being 105 m apart, which could be related to the kind of seed dispersal promoted by Brachyteles arachnoides (Primates - Cebidae). Results showed that for an adequate sampling, aiming to detect genetic diversity, the collection of individuals separated by a distance of 750 m, or more, from each other, would be advisable.
Descrição
Palavras-chave
Análise multilocos, autocorrelação espacial, coeficiente I de Moran, Cryptocarya moschata, isoenzimas, Cryptocarya moschata, isozymes, Moran's I coefficients, multilocus analysis, spatial autocorrelation
Como citar
Brazilian Journal of Botany. Sociedade Botânica de São Paulo, v. 27, n. 3, p. 475-487, 2004.