Respostas endócrinas e ovarianas associadas com o folículo dominante da primeira onda folicular em ovelhas sincronizadas com CIDR ou PGF2alfa
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Data
2002-04-01
Autores
Uribe-Velásquez, Luis Fernando
Oba, Eunice [UNESP]
Lara-Herrera, Lida Constanza [UNESP]
Souza, Maria Inês Lenz [UNESP]
Villa-Velásquez, Hooverman [UNESP]
Trinca, Luzia Aparecida [UNESP]
Fernandes, Carlos Antônio de Carvalho
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Zootecnia
Resumo
Os efeitos de prostaglandina (PGF2a) vs CIDR e eCG (gonadotrofina coriônica eqüina) na dinâmica folicular da primeira onda e sua relação com as concentrações plasmáticas de P4 e E2 foram investigadas em ovelhas cíclicas. Foram utilizadas 14 fêmeas ovinas da raça Bergamascia; o Grupo 1 (G1) foi submetido a duas aplicações de PGF2alfa e o Grupo 2 (G2), tratado com CIDR durante 14 dias, sendo que, no momento de sua retirada, administraram-se 500 UI de eCG. A dinâmica folicular ovariana foi monitorada por meio de ultra-som. Monitoraram-se todos os folículos > 2mm e mapeou-se sua posição diariamente, observando-se o desenvolvimento individual de cada folículo. Desde o dia anterior à aplicação da segunda dose de PGF2alfa (G1) e desde a administração de eCG (G2) até o décimo dia do ciclo estral, foram coletadas amostras de sangue para análise de P4 e E2. Houve diferença significativa nas concentrações plasmáticas de P4 e E2 entre os tratamentos. A sincronização de estro e ovulação, utilizando CIDR + 500 UI de eCG, incrementou a quantidade de folículos recrutados, além de aumentar o diâmetro máximo e a taxa de crescimento dos folículos grandes na primeira onda folicular.
The effect of prostaglandin (PGF2a) vs CIDR and eCG (equine chorionic gonadotrophin) on follicular dynamic of wave 1 and its relation with P4 and E2 plasma concentrations were investigated in cyclic ewes. Fourteen Bergamascia ewes were used, Group 1 (G1) synchronized by two i.m. injections of PGF2alpha and Group 2 (G2) treated for 14 d with CIDR and 500 IU of eCG at dispositive withdrawal on day 14. Ovarian follicular dynamics were ultrasonically monitored. All the ovarian follicles > 2 mm were measured, and their relative locations were recorded on an ovarian map in order to follow the sequential development of each individual follicle. Blood samples for P4 and E2 determination were collected daily from 1 d before the second injection of PGF2alpha (G1) and administration of eCG (G2) until day 10 after ovulation of the cycle. There were significant differences in P4 and E2 plasma concentrations between treatments. The CIDR + eCG (500 IU) treatment enhanced the recruitment of smaller follicles and enhanced the maximum diameter and growth rate of large follicles during wave 1.
The effect of prostaglandin (PGF2a) vs CIDR and eCG (equine chorionic gonadotrophin) on follicular dynamic of wave 1 and its relation with P4 and E2 plasma concentrations were investigated in cyclic ewes. Fourteen Bergamascia ewes were used, Group 1 (G1) synchronized by two i.m. injections of PGF2alpha and Group 2 (G2) treated for 14 d with CIDR and 500 IU of eCG at dispositive withdrawal on day 14. Ovarian follicular dynamics were ultrasonically monitored. All the ovarian follicles > 2 mm were measured, and their relative locations were recorded on an ovarian map in order to follow the sequential development of each individual follicle. Blood samples for P4 and E2 determination were collected daily from 1 d before the second injection of PGF2alpha (G1) and administration of eCG (G2) until day 10 after ovulation of the cycle. There were significant differences in P4 and E2 plasma concentrations between treatments. The CIDR + eCG (500 IU) treatment enhanced the recruitment of smaller follicles and enhanced the maximum diameter and growth rate of large follicles during wave 1.
Descrição
Palavras-chave
dinâmica folicular, estradiol (E2), ovinos, progesterona (P4), ultra-sonografia, follicular dynamics, oestradiol (E2), ovine, progesterone (P4), ultrasonography
Como citar
Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 31, n. 2, p. 944-953, 2002.