Opposite effects of bFGF and TGF-beta on collagen metabolism by human periodontal ligament fibroblasts
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Elsevier B.V.
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This study evaluated the effects of bFGF and TGF-beta, individually and combined, on cell proliferation and collagen metabolism. Primary human periodontal ligament cells were stimulated with two concentrations (I and 10 ng/ml) of each growth factor, both individually and combined. Proliferation was determined by a commercial biochemical assay. Real time RT-PCR determined gene expression of NMP-1 and -2, collagen types I and III, TIMP-1, -2 and -3. Autocrine effects on synthesis of bFGF and TGF-beta were evaluated by ELISA. Only TGF-beta, either isolated or associated with bFGF, significantly increased cell proliferation. TGF-beta had anabolic effects, increasing expression of type I and III collagen as well as of TIMPs, whereas bFGF had opposite effects. When bFGF and TGF-beta were associated, the anabolic effects prevailed. Synthesis of TGF-beta was induced only by the association of lower concentrations of the growth factors, whereas there was a dose-dependent production of bFGF. It is concluded that bFGF had a predominantly catabolic effect, and TGF-beta exerted an anabolic effect on hPDL cells. (c) 2007 Elsevier Ltd. All rights reserved.
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human periodontal ligament cells, transforming growth factor beta, basic fibroblast growth factor, collagen, matrix metalloproteases, tissue inhibitor of matrix metalloproteases, periodontal regeneration
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Inglês
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Cytokine. London: Academic Press Ltd Elsevier B.V. Ltd, v. 39, n. 2, p. 130-137, 2007.
