Publicação: Polystyrene-type resin used for peptide synthesis: application for anion-exchange and affinity chromatography
Nenhuma Miniatura disponível
Data
2005-03-25
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Tipo
Artigo
Direito de acesso
Acesso restrito
Resumo
This paper deals with an unusual application for a copolymer of styrene-1 % divinylbenzene bearing high amount of aminomethyl groups for anion-exchange and affinity chromatography. The so-called aminomethyl resin (AMR), to date only employed for peptide synthesis, swelled appreciably in water and was used successfully to purify negatively charged peptides. By correlating swelling degree of beads with pH of the media, it was possible to estimate that the AMR amino group pK(a) is approximately 5.5. In addition, the synthesized acetyl-(NANP)(3)-AMR succeeded in the affinity interaction with large antibody molecules related to malaria transmission and raised previously against this dodecapeptide sequence. (C) 2004 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
Idioma
Inglês
Como citar
Journal of Chromatography B-analytical Technologies In the Biomedical and Life Sciences. Amsterdam: Elsevier B.V., v. 817, n. 2, p. 231-238, 2005.
