Polymorphism of the ITS-2 region of the ribosomal DNA of the Triatominae Rhodnius domesticus, R-pictipes, R-prolixus and R-stali
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Data
2006-12-01
Autores
Naegele, M. P.
Costa, Paulo Inácio da [UNESP]
Rosa, João Aristeu da [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Blackwell Publishing
Resumo
The polymerase chain reaction-restriction fragment length polymorphism technique (PCR-RFLP) was used to compare Rhodnius domesticus (Neiva & Pinto), R. pictipes (Stal), R. prolixus (Stal) and R. stali (Lent; Jurberg & Galvao) (Hemiptera: Reduviidae). The enzyme BstUI differentiated R. donzesticus, R. pictipes and R. prolixus, and HhaI differentiated R. domesticus, R. pictipes and R. stali. With the fingerprinting analysis generated by these two enzymes, it was possible to clearly identify all four species in the study.
Descrição
Palavras-chave
Rhodnius, endonucleases, ITS-2, PCR-RFLP, Triatominae
Como citar
Medical and Veterinary Entomology. Oxford: Blackwell Publishing, v. 20, n. 4, p. 353-357, 2006.