Natural infection with zoonotic subtype of Cryptosporidium parvum in Capybara (Hydrochoerus hydrochaeris) from Brazil

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Data

2007-06-20

Autores

Vasconcelos Meireles, Marcelo
Martins Soares, Rodrigo
Bonello, Fabio
Maria Gennari, Solange

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Editor

Elsevier B.V.

Resumo

A total of 145 capybara (Hydrochoerus hydrochaeris) fecal samples from the state of Sdo Paulo, Brazil, were screened for Cryptosporidium spp. oocysts using the malachite green method. Eight samples (5.52%) showed positive results and were further submitted to nested PCR reaction for amplification of fragments of 18S rRNA gene and 60-kDa glycoprotein gene for determination of species, alleles and subtypes of Cryptosporidium. Sequencing of the PCR products of the 18S rRNA gene fragments and 60-kDa glycoprotein gene fragments showed that for both genes all Cryptosporidium isolates from capybara were respectively 100% genetically similar to a bovine isolate of C. parvum and to C parvum subtype IIaA15G2R1. To the best of our knowledge this is the first report of Cryptosporidium infection in this rodent. The finding of zoonotic C parvum infection in a semi-aquatic mammal that inhabits anthroponotic habitats raises the concern that human water supplies may be contaminated with zoonotic Cryptosporidium oocysts from wildlife. (c) 2007 Elsevier B.V. All rights reserved.

Descrição

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Cryptosporidium, Hydrochoerus hydrochaeris, 60-kDa glycoprotein gene, 18S rRNA gene, natural infection

Como citar

Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 147, n. 1-2, p. 166-170, 2007.