Publicação: Partial purification and some properties of a T2 ribonuclease from Aspergillus flavipes
Carregando...
Data
Autores
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Faculty Press
Tipo
Artigo
Direito de acesso
Acesso restrito
Resumo
A ribonuclease was partially purified from the culture medium of Aspergillus flavipes (IZ:1501), after 96 h of cultivation by chromatography on DEAE-cellulose and Sephadex G100 columns. The molecular weight of the RNase was estimated to be 40 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 50-55 degrees C, respectively. Catalytic activity was inhibited by Zn+2, Fe+3, Hg+2 and Ag+ ions. The enzyme did not show an exact base specificity and produced four kinds of 3'-nucleotides from yeast RNA.
Descrição
Palavras-chave
ribonuclease, Aspergillus, nucleotides
Idioma
Inglês
Como citar
Microbios. Cambridge: Faculty Press, v. 87, n. 353, p. 227-237, 1996.
