The dead core model applied to beads with immobilized cells in a fed-batch cephalosporin C production bioprocess
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Data
2001-01-01
Autores
Cruz, AJG
Almeida, RMRG
Araujo, MLGC
Giordano, R. C.
Hokka, C. O.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Viable cells immobilized in inert supports are currently studied for a wide range of bioprocesses. The intrinsic advantages of such systems over suspended cultures incite new research, including studies on fundamental aspects as well as on the industrial viability of these non-conventional processes. In aerobic culture of filamentous fungi, scale-up is hindered by oxygen mass transfer limitation through the support material and bioprocess kinetics must be studied together with mass transfer limitation. In this work, experimental and simulated data of cephalosporin C production were compared. Concentrations in the bulk fermentation medium and cellular mass profiles inside the bioparticles are focused. Immobilized cells were used in a tower bioreactor, operated in fed-batch mode. To describe the radial variation of oxygen concentration within the pellet, a dead core model was used. Despite the extremely low sugar concentrations, bioreaction rates in the pellets were limited by the dissolved oxygen concentration. Cell growth occurs only in the outer layers, a result also confirmed by scanning electron microscopy. (C) 2001 Elsevier B.V. Ltd. All rights reserved.
Descrição
Palavras-chave
cephalosporin C, beta-lactam, Cephalosporium acremonium, dead core model, fed-batch
Como citar
Chemical Engineering Science. Oxford: Pergamon-Elsevier B.V., v. 56, n. 2, p. 419-425, 2001.