Application of synthetic peptides to the diagnosis of neurocysticercosis
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Data
2003-12-01
Autores
Fleury, A.
Beltran, C.
Ferrer, E.
Garate, T.
Harrison, LJS
Parkhouse, RME
Garcia, E.
Fragoso, G.
Costa-Cruz, J.
Biondi, G.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Blackwell Publishing
Resumo
We tested the possible diagnostic utility of five Taenia saginata oncosphere-derived synthetic peptides in T. solium neurocysticercosis (NC). The five peptides correspond to protein sequences with high antigenic indexes that were cloned from a T. saginata oncosphere cDNA library. The test samples consisted of cerebrospinal fluid (CSF) samples randomly collected from patients referred from Mexican and Brazilian neurological institutes. Indirect enzyme-linked immunosorbent assays (ELISA) were carried out with the peptides either unconjugated or coupled to carrier proteins, and were compared with results obtained using T. solium cyst fluid as a positive control. For active inflammatory NC, the higher sensibility (93%) and specificity (85%) was obtained with peptides HP6-2 and Ts45W-1, respectively, coupled to ovalbumin, in both Mexican and Brazilian patients. Examining the results of the individual peptide assays in combination, in some instances, improved the sensitivity to 100%.
Descrição
Palavras-chave
peptides, Taenia solium, neurocysticercosis, diagnosis
Como citar
Tropical Medicine & International Health. Oxford: Blackwell Publishing Ltd, v. 8, n. 12, p. 1124-1130, 2003.