Computer-assisted analysis of cell proliferation markers in oral lesions
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Data
2007-01-01
Autores
Teresa, Debora Barreto
Neves, Karina Antunes
Neto, Carlos Benatti
Fregonezi, Paula Andrea Gabrielli
de Oliveira, Maria Rita Brancini
Zuanon, Jose Antonio Sampaio
Donadi, Eduardo Antonio
Mendes, Celso Texixeira
Soares, Christiane Pienna [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Abnormalities in any component of the cell cycle regulatory machine may result in oral. cancer, and markers of cell proliferation have been used to determine the prognosis of tumor progression. The aim of this study was to determine whether silver-stained nucleolar organizer region (AgNOR) and Ki-67 measurements could improve the assessment of growth rates in oral lesions. Eighty-three oral biopsies were studied, 20 of which were classified as fibrous inflammatory hyperplasia (FIH), 40 as leukoplakia (LKP) and 23 as oral. squamous cell carcinoma (OSCC). Within the LKP group, 22 out of 29 biopsies were diagnosed as non-dysplastic leukoplakia (LK) and 18 as dysplastic teukoptakia (DLK), presenting discrete, moderate and severe dysplasia. Ki-67 immunotabeting of the lesions increased steadily in the following order: FIH, DLK, LK and OSCC, indicating that Ki-67 is a good marker for predicting the protiferative fraction among benign, premalignant and malignant oral lesions. The median values of AgNOR parameters indicate that the morphometric index gives better results regarding the proliferative rate than the numerical one. A series of linear regressions between AgNOR parameters and Ki-67 showed positive associations. We conclude that a combination of Ki-67 and morphometric AgNOR analyses could be used as an aid in the determination of the protiferative status of oral epithelial. cells in oral cancer. (C) 2007 Elsevier GmbH. All rights reserved.
Descrição
Palavras-chave
oral cancer, immunohistochemistry, ki-67 antigen, AgNOR, computer-assisted system, image analysis, proliferative markers
Como citar
Acta Histochemica. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 109, n. 5, p. 377-387, 2007.