Gene discovery in Boophilus microplus, the cattle tick - the transcriptomes of ovaries, salivary glands, and hemocytes
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Data
2004-01-01
Autores
Santos, IKFD
Valenzuela, J. G.
Ribeiro, JMC
De Castro, M.
Costa, J. N.
Costa, A. M.
Da Silva, E. R.
Neto, OBR
Rocha, C.
Daffre, S.
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New York Acad Sciences
Resumo
The quest for new control strategies for ticks can profit from high throughput genomics. In order to identify genes that are involved in oogenesis and development, in defense, and in hematophagy, the transcriptomes of ovaries, hemocytes, and salivary glands from rapidly ingurgitating females, and of salivary glands from males of Boophilus microplus were PCR amplified, and the expressed sequence tags (EST) of random clones were mass sequenced. So far, more than 1,344 EST have been generated for these tissues, with approximately 30% novelty, depending on the the tissue studied. To date approximately 760 nucleotide sequences from B. microplus are deposited in the NCBI database. Mass sequencing of partial cDNAs of parasite genes can build up this scant database and rapidly generate a large quantity of useful information about potential targets for immunobiological or chemical control.
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Boophilus microplus, transcriptomes, salivary glands, ovary, hemocytes
Como citar
Impact of Ecological Changes on Tropical Animal Health and Disease Control. New York: New York Acad Sciences, v. 1026, p. 242-246, 2004.