Clavulanic acid production by immobilized cells if Streptomyces clavuligerus
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Data
2006-02-01
Autores
Sperandei Lavarda, Susana Cristina
Hokka, Carlos Osamu
Gonsales da CostaAraujo, Maria Lucia
Título da Revista
ISSN da Revista
Título de Volume
Editor
Inst Tecnologia Parana
Resumo
A clavulanic acid production process with immobilized Streptomyces clavuligerus cells was investigated. Cells were immobilized in diatomaceous earth, calcium alginate gel as well as in the form of natural pellets and cultivated in shake flasks in a medium containing glycerol and soytone as the carbon and nitrogen sources, respectively. In all experiments growth occurred in the first 48 h and glycerol consumption after 72 h, while clavulanic acid production was observed between 48 and 60h, with gradual degradation after this period. The natural pellets presented higher product concentration as compared with the cells immobilized in supports. However, calcium alginate was found to be the best support in relation to cell retention capacity.
Descrição
Palavras-chave
alginate calcium gel, celite, immobilization cells, pellets, Streptomyces clavuligerus
Como citar
Brazilian Archives of Biology and Technology. Curitiba-Paraná: Inst Tecnologia Parana, v. 49, p. 125-131, 2006.