Publicação: Electrochemical measurements of oligonucleotides in the presence of chromosomal DNA using membrane-covered carbon electrodes
Nenhuma Miniatura disponível
Data
1997-10-01
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Amer Chemical Soc
Tipo
Artigo
Direito de acesso
Acesso restrito
Resumo
Substantial improvements in the selectivity of electrochemical measurements of trace nucleic adds are obtained by using membrane-covered carbon disk electrodes. Access to the electrode surface can be manipulated via a judicious choice of the membrane molecular weight cutoff (MWCO). The resulting separation step, performed in situ at the electrode surface, adds a new dimension of selectivity based on molecular size to electroanalysis of nucleic acids, Transport properties are evaluated with respect to the oligonucleotide length and membrane MWCO. A highly selective response is observed for synthetic oligonucleotides in the presence of otherwise interfering chromosomal DNAs. Discrimination among oligonucleotides of different lengths is also possible, Short accumulation periods (1-5 min) are sufficient for convenient measurements of low milligram per liter concentrations.
Descrição
Palavras-chave
Idioma
Inglês
Como citar
Analytical Chemistry. Washington: Amer Chemical Soc, v. 69, n. 19, p. 4056-4059, 1997.
