Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg
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2008-12-01
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Brazilian Society of Infectious Diseases
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The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. It is the first description of a lettuce Brazilian variety Vitória de Verão genetically modified.
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Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 12, n. 6, p. 469-471, 2008.