Respostas Fisiológicas Associadas à Termotolerância em Pintos de Corte de Duas Linhagens por Exposição a Altas Temperaturas
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Data
2002-02-01
Autores
Dionello, Nelson José Laurino
Macari, Marcos [UNESP]
Ferro, Jesus Aparecido [UNESP]
Rutz, Fernando
Ferro, Maria Inês Tiraboschi [UNESP]
Furlan, Luiz Roberto
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Zootecnia
Resumo
Com o objetivo de se avaliar o comportamento fisiológico das aves, bem como, se ocorre a expressão da proteína do choque térmico (Hsp 70) no fígado e cérebro, como conseqüência da exposição ao estresse por calor, em quatro dias sucessivos (2 a 5 dias de vida), 20 pintos de corte de duas linhagens Pescoço pelado (Na/na) e Hubbard-Petersen, foram ou não estressados à temperatura de 36-37°C, durante cinco horas. Foram colhidas amostras de fígado e cérebro, ao final do período de estresse, e analisadas por Western Blotting, para determinação dos níveis de Hsp 70. Os resultados não mostraram associação entre perda de peso corporal e as demais características. Aumentos da variação de temperatura cloacal apresentaram aumentos nos níveis de Hsp70 no tecido hepático e cerebral. Concluiu-se que a concentração de Hsp70 no cérebro, apresentou-se correlacionada com a variação de temperatura cloacal, quando se usou o condicionamento ao calor em pintos de corte, embora não se possa afirmar que seria indicativo de termotolerância de aves.
This study was run to evaluate the physiological response and expression of hepatic and brain Hsp70, of 20 Naked neck (Na/na) and Hubbard-Petersen broiler chicks (2 to 5 days of age), exposed or not to 36-37°C. The trial was conducted during four days and the birds were exposed to high temperature during 5 hours. Liver and brain tissue samples were collected at the end of the stress period and analyzed for Western Blotting for determination of Hsp70 level. No association between body weight loss and the other variables were observed. Increasing the variation in cloacal temperature resulted in an increase in Hsp70 hepatic and brain tissue levels. Results indicate that brain Hsp70 levels were correlated with variation in cloacal temperature, concerning the chicks subjected to heat stress, but it can not be stated that it is an indicative of thermotolerance by the birds.
This study was run to evaluate the physiological response and expression of hepatic and brain Hsp70, of 20 Naked neck (Na/na) and Hubbard-Petersen broiler chicks (2 to 5 days of age), exposed or not to 36-37°C. The trial was conducted during four days and the birds were exposed to high temperature during 5 hours. Liver and brain tissue samples were collected at the end of the stress period and analyzed for Western Blotting for determination of Hsp70 level. No association between body weight loss and the other variables were observed. Increasing the variation in cloacal temperature resulted in an increase in Hsp70 hepatic and brain tissue levels. Results indicate that brain Hsp70 levels were correlated with variation in cloacal temperature, concerning the chicks subjected to heat stress, but it can not be stated that it is an indicative of thermotolerance by the birds.
Descrição
Palavras-chave
Broiler chicks, cloacal temperature, Heat stress, liver and brain Hsp70 levels, Hsp70 hepática e cerebral, Estresse por calor, temperatura cloacal, Frangos de corte
Como citar
Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 31, n. 1, p. 79-85, 2002.