Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
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Data
2012-11-01
Autores
David, S.
Sutre, A. F.
Sanca, A.
Mane, A.
Henriques, V.
Portugal, C.
Sancho, L.
Cardoso, A.
Paixao, E.
Duarte, E. L.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Int Union Against Tuberculosis Lung Disease (i U A T L D)
Resumo
The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair ppoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.
Descrição
Palavras-chave
Mycobacterium tuberculosis, rpoB, sputum smear microscopy, sodium hypochlorite, denaturing high-performance liquid chromatography (DHPLC) sequencing
Como citar
International Journal of Tuberculosis and Lung Disease. Paris: Int Union Against Tuberculosis Lung Disease (i U A T L D), v. 16, n. 11, p. 1535-1537, 2012.