Validation of a Leishmania infantum ELISA rapid test for serological diagnosis of Leishmania chagasi in dogs
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Data
2011-01-10
Autores
Marcondes, M. [UNESP]
Biondo, A. W.
Gomes, A. A. D. [UNESP]
Silva, A. R. S. [UNESP]
Vieira, R. F. C.
Camacho, Aparecido Antonio [UNESP]
Quinn, John
Chandrashekarg, R.
Título da Revista
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Editor
Elsevier B.V.
Resumo
Canine visceral leishmaniasis (CVL) is caused by Leishmania donovani complex parasites including L donovani, Leishmania infantum and Leishmania chagasi. As some studies suggest that L. chagasi and L infantum may be very similar or even the same species, the aim of the present study was to evaluate a commercial rapid ELISA test, originally designed for L. infantum, in the diagnosis of CVL in dogs naturally infected by L chagasi. A total of 400 serum canine samples, including 283 positive dogs for CVL from an endemic area, 86 clinically healthy dogs from a non-endemic area and 31 dogs seropositive for confounding infectious agents (Trypanosoma cruzi,Toxoplasma gondii, Neospora caninum, Babesia canis and Ehrlichia canis) were used for test validation. An overall sensitivity of 94.7% (95% CI = 91.41-97.01%) and specificity of 90.6% (95% CI = 83.80-95.21%) was found, with a high degree of agreement (k = 0.8445) to the indirect ELISA. When confounding infectious diseases were excluded, specificity increased to 100% (95% CI = 95.8-100%), with a higher degree of agreement (k = 0.8928). In conclusion, the commercial kit designed for L infantum was a highly sensitive and specific device for detection of L chagasi infection in dogs, which indicates high immunoreactivity similarities between L infantum and L chagasi. (C) 2010 Elsevier B.V. All rights reserved.
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Palavras-chave
Visceral Leishmaniasis, Serology, Snap
Como citar
Veterinary Parasitology. Amsterdam: Elsevier B.V., v. 175, n. 1-2, p. 15-19, 2011.