Concentração da solução de tetrazólio e período de coloração do teste para sementes de mamoneira
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Data
2009-01-01
Autores
Gaspar-Oliveira, Carolina Maria
Martins, Cibele Chalita [UNESP]
Nakagawa, João [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Associação Brasileira de Tecnologia de Sementes (ABRATES)
Resumo
O objetivo do trabalho foi estudar a concentração da solução de tetrazólio e o período de coloração do teste para a avaliação do potencial fisiológico de sementes de mamoneira (Ricinus communis L.), padronizando a nomenclatura das cores observadas nas sementes após a coloração. Os tratamentos de concentração da solução de tetrazólio e períodos de coloração estudados foram: 0,075% e 0,1% por 120, 180 e 240 minutos, 0,2% por 60, 120 e 180 minutos, 0,5% por 60, 90 e 120 minutos e 1,0% por 30, 60 e 90 minutos. Os resultados foram comparados com os obtidos nos testes de germinação. A coloração das sementes após o teste de tetrazólio, em cada tratamento, foi avaliada mediante comparação com as fichas de cor do catálogo de Munsell, determinando-se a porcentagem de sementes observada em cada cor. O delineamento experimental foi o inteiramente casualizado e a comparação de médias realizada pelo teste de Tukey a 5% de probabilidade. Para avaliar o potencial fisiológico pelo teste de tetrazólio, as sementes de mamoneira devem ser imersas na solução de tetrazólio na concentração de 0,2% por 120 minutos, a 35ºC. Nesse tratamento, as sementes viáveis após a coloração na solução de tetrazólio apresentaram predominantemente as cores rosa e rosa-escuro em suas estruturas essenciais, portanto essas podem ser consideradas como as cores características para o teste de tetrazólio em sementes de mamoneira.
The objective of this research was to study the tetrazolium solution concentration and staining period for the evaluation of the physiological quality of castor bean seeds (Ricinus communis L.) by the tetrazolium test, standardizing the nomenclature of the observed colors in seeds after staining. The treatments of tetrazolium solution concentration and staining period were: 0.075% and 0.1% for 120, 180 and 240 minutes, 0.2% for 60, 120 and 180 minutes, 0.5% for 60, 90 and 120 minutes and 1.0% for 30, 60 and 90 minutes. The results were compared to the germination test. The seed color after the tetrazolium test in each treatment was evaluated by comparing with the Munsell book of color, and the percentage of seeds observed in each color was established. A randomized complete block design was used and the means were compared by the Tukey test at the 0.05 level of probability. To evaluate physiological quality by the tetrazolium test, castor bean seeds should be placed in tetrazolium solution at the concentration 0.2% for 120 minutes, at 35ºC, for staining development, and in this treatment, the viable seeds after the staining in tetrazolium solution showed predominantly the colors pink and dark pink in their essential structures. Thus these colors can be considered as the characteristics colors for the tetrazolium test in castor bean seeds.
The objective of this research was to study the tetrazolium solution concentration and staining period for the evaluation of the physiological quality of castor bean seeds (Ricinus communis L.) by the tetrazolium test, standardizing the nomenclature of the observed colors in seeds after staining. The treatments of tetrazolium solution concentration and staining period were: 0.075% and 0.1% for 120, 180 and 240 minutes, 0.2% for 60, 120 and 180 minutes, 0.5% for 60, 90 and 120 minutes and 1.0% for 30, 60 and 90 minutes. The results were compared to the germination test. The seed color after the tetrazolium test in each treatment was evaluated by comparing with the Munsell book of color, and the percentage of seeds observed in each color was established. A randomized complete block design was used and the means were compared by the Tukey test at the 0.05 level of probability. To evaluate physiological quality by the tetrazolium test, castor bean seeds should be placed in tetrazolium solution at the concentration 0.2% for 120 minutes, at 35ºC, for staining development, and in this treatment, the viable seeds after the staining in tetrazolium solution showed predominantly the colors pink and dark pink in their essential structures. Thus these colors can be considered as the characteristics colors for the tetrazolium test in castor bean seeds.
Descrição
Palavras-chave
viabilidade, mamona, metodologia, coloração, viability, methodology, color
Como citar
Revista Brasileira de Sementes. Associação Brasileira de Tecnologia de Sementes, v. 31, n. 3, p. 38-47, 2009.