Protein synthesis studies in skeletal muscle of aging rats. II. In vitro studies with 0.5M potassium chloride washed polyribosomes

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Data

1984-01-01

Autores

Burini, Roberto Carlos [UNESP]
Pluskal, M. G.
Wei, I.
Young, V. R.

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Resumo

To investigate further the age-related reduction in muscle protein synthesis activity found previously using a crude polyribosome/pH 5 system (Pluskal et al., 1984), a 0.5M KCl washing procedure was utilized to remove the nonribosomal factors from polyribosomes isolated from male Sprague-Dawley rats in the following age groups: young (1 to 2 months), mature (12 months), and aged (22 to 24 months). Using a common source of enriched elongation factor fraction from young animals, it was not possible to demonstrate any significant difference (p > .05) in protein synthesis between the 0.5M KCl-washed polyribosomes isolated from the various age groups. Using a cell-free system containing young salt washed polyribosomes stimulated by the addition of 0.5M KCl-wash fractions, however, it was shown that the mature and aged salt-wash fractions were less (p < .05) active than material from young animals. Thus, the observed decline in protein synthesis efficiency during aging may be attributed to a reduced capacity to promote initiation/elongation by the nonribosomal salt wash fractions of muscle polyribosomes.

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Palavras-chave

age, aging, animal experiment, cytology, muscle, nonhuman, polysome, protein synthesis, rat, Aging, Amino Acids, Animal, Cell-Free System, In Vitro, Male, Polyribosomes, Potassium Chloride, Proteins, Rats, Rats, Inbred Strains, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S.

Como citar

Journals of Gerontology, v. 39, n. 4, p. 392-397, 1984.