Protein synthesis studies in skeletal muscle of aging rats. II. In vitro studies with 0.5M potassium chloride washed polyribosomes
Carregando...
Arquivos
Fonte externa
Fonte externa
Data
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Tipo
Artigo
Direito de acesso
Acesso restrito
Arquivos
Fonte externa
Fonte externa
Resumo
To investigate further the age-related reduction in muscle protein synthesis activity found previously using a crude polyribosome/pH 5 system (Pluskal et al., 1984), a 0.5M KCl washing procedure was utilized to remove the nonribosomal factors from polyribosomes isolated from male Sprague-Dawley rats in the following age groups: young (1 to 2 months), mature (12 months), and aged (22 to 24 months). Using a common source of enriched elongation factor fraction from young animals, it was not possible to demonstrate any significant difference (p > .05) in protein synthesis between the 0.5M KCl-washed polyribosomes isolated from the various age groups. Using a cell-free system containing young salt washed polyribosomes stimulated by the addition of 0.5M KCl-wash fractions, however, it was shown that the mature and aged salt-wash fractions were less (p < .05) active than material from young animals. Thus, the observed decline in protein synthesis efficiency during aging may be attributed to a reduced capacity to promote initiation/elongation by the nonribosomal salt wash fractions of muscle polyribosomes.
Descrição
Palavras-chave
age, aging, animal experiment, cytology, muscle, nonhuman, polysome, protein synthesis, rat, Aging, Amino Acids, Animal, Cell-Free System, In Vitro, Male, Polyribosomes, Potassium Chloride, Proteins, Rats, Rats, Inbred Strains, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S.
Idioma
Inglês
Citação
Journals of Gerontology, v. 39, n. 4, p. 392-397, 1984.
