Chromosome damage induced by 5-azacytidine under the influence of caffeine or cytosine arabinoside in CHO-K1 (wild-type) and XRS-5 (mutant) cell lines
Nenhuma Miniatura disponível
Data
2001-07-14
Autores
Sakamoto-Hojo, E. T.
Bicego-Nahas, K. C.
Takahashi-Hyodo, S. A.
Tavares, D. C. [UNESP]
Dias, F. L.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
5-azacytidine (5-azaC) treatment combined with cytosine arabinoside (ara-C) or caffeine were performed in vitro in Chinese hamster cells, CHO-K1 (wild-type) and xrs-5 (mutant) cell lines, in order to compare the cell response to the induction of chromosomal aberrations. Exponentially growing cells were treated with 5-azaC (4-16 uM) for 1 h, the cells were washed and incubated for 7 h, and 500 uM caffeine or 5 uM ara-C were added to the cultures for the last 2 h. In both cell lines, 5-azaC induced a significantly increase (P<0.01) in the frequencies of aberrations; in the combined treatments (5-azaC + Ara-C), a significant reduction (P<0.05) was observed for the aberrations which were randomly distributed. Caffeine had no influence at the same conditions. 5-azaC induced-DNA lesions were probably processed at S/G2 phase in a common pathway in both cell lines, but alternatively, 5-azaC may cause xrs-5 cells to revert to the wild-type.
Descrição
Palavras-chave
5-Azacytidine, Caffeine, Chromosomal aberrations, Cytosine arabionside, azacitidine, caffeine, cytarabine, animal cell, cell cycle G2 phase, cell cycle S phase, cell growth, cell line, CHO cell, chromosome aberration, chromosome damage, controlled study, nonhuman, priority journal
Como citar
Cancer Research, Therapy and Control, v. 11, n. 2, p. 125-134, 2001.