Cromatografia em contra-corrente de gotejamento na separação de substâncias fenólicas de Neuropogon aurantiaco-ater
Título alternativoDroplet counter-current chromatography in the separation of phenolic substances from Neuropogon aurantiaco-ater
Data de publicação2001-12-01
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This work reports the separation of phenolic compounds present in the metabolic extract of the lichen Neuropogon aurantiaco-ater (Usniaceae). The crude extract was fractionated by droplet counter-current chromatography using a solvent mixture of high polarity (chloroform:methanol:water at 43:37:20, in ascending mode). The separation resulted in the isolation of usnic and protocetratic acid, which were identified by TLC, HPLC, and NMR tests.