Molecular typing of IberoAmerican Cryptococcus neoformans isolates
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2003-02-01
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A network was established to acquire basic knowledge of Cryptococcus neoformans in IberoAmerican countries. To this effect, 340 clinical, veterinary, and environmental isolates from Argentina, Brazil, Chile, Colombia, Mexico, Peru, Venezuela, Guatemala, and Spain were typed by using M13 polymerase chain reaction-fingerprinting and orotidine monophosphate pyrophosphorylase (URA5) gene restriction fragment length polymorphsm analysis with Hhal and Sau961 in a double digest. Both techniques grouped all isolates into eight previously established molecular types. The majority of the isolates, 68.2% (n=232), were VNI (var. grubii, serotype A), which accords with the fact that this variety causes most human cryptococcal infections worldwide. A smaller proportion, 5.6% (n=19), were VNII (var. grubii, serotype A); 4.1% (n=14), VNIII (AD hybrid), with 9 isolates having a polymorphism in the URA5 gene; 1.8% (n=6), VNIV (var. neoformans, serotype D); 3.5% (n=12), VGI; 6.2% (n=21), VGII; 9.1% (n=31), VGIII, and 1.5% (n=5) VGIV, with all four VG types containing var. gattii serotypes B and C isolates.
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controlled study , Cryptococcus neoformans , DNA fingerprinting , DNA polymorphism , fungal gene , fungus isolation , genetic analysis , geographic distribution , molecular typing , nonhuman , polymerase chain reaction , restriction fragment length polymorphism , serotype , ura5 gene , virus gene , Adult , Aged , Animals , Bacterial Typing Techniques , Central America , Cryptococcosis , DNA Fingerprinting , Epidemiology, Molecular , Female , Goats , Humans , Orotate Phosphoribosyltransferase , Parrots , Phylogeny , Polymorphism, Genetic , Polymorphism, Restriction Fragment Length , South America , Spain
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Inglês
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Emerging Infectious Diseases, v. 9, n. 2, p. 189-195, 2003.