Quantification of sex pheromone from Anocentor nitens by gas chromatography-mass spectrometry-selected ion monitoring
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Data
2004-02-27
Autores
Rodrigues, Clenilson M. [UNESP]
Borges, Lígia M.F.
Ferri, Pedro H.
Faria, Lourival C.
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Resumo
Two highly sensitive and selective methods based on gas chromatography coupled to mass spectrometry (GC-MS) in the selected ion monitoring (SIM) mode have been developed for the quantification of 2,6-dichlorophenol (2,6-DCP), a sex pheromone of the tick females of Anocentor nitens. Standard addition method and calibration curve techniques using 5-bromine-4-hydroxy-3- methoxybenzaldehyde (5-BrV) as internal standard (IS) afforded detection limit of 0.1ngml-1. The calibration curve was linear over the concentration range from 0.5 to 500ngml-1 for 2,6-DCP. Results show that the concentration range of sex pheromone in the extracts samples was 1.08-10.35ngml-1. The methods developed provided reliable procedures to determine amounts of 2,6-DCP present in ticks. © 2003 Elsevier B.V. All rights reserved.
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2,6-Dichlorophenol, Anocentor nitens, GC-MS-SIM, Calibration, Gas chromatography, Mass spectrometry, Detection limits, Phenols, 2,6 dichlorophenol, 5 bromine 4 hydroxy 3 methoxybenzaldehyde, benzaldehyde derivative, sex pheromone, unclassified drug, calibration, controlled study, extraction, female, gas chromatography, hormone determination, ion monitoring, mass spectrometry, nonhuman, quantitative analysis, sex difference, standard, technique, tick
Como citar
Talanta, v. 62, n. 3, p. 637-641, 2004.