Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis
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Paired samples of cerebrospinal fluid (CSF) and serum of 30 patients - 10 with active, 10 with inactive neurocysticercosis (NCC), and 10 control subjects - were evaluated by enzyme-linked immunosorbent assay (ELISA) using two Taenia crassiceps metacestode extracts as antigen in order to detect IgG antibodies. In active NCC, high levels of IgG were detected (p < 0.05). The CSF samples showed 80% (CI 72-88) of reactivity in the saline extract (S) and 90% (CI 84-95) in sodium dodecyl sulphate (SDS) and the serum samples were reactive in 90% (CI 84-95) and 100% (CI 98-100) in the S and SDS antigenic extracts, respectively. The use of the paired samples of CSF and serum in active NCC showed equivalent results suggesting that the serum samples could be used as a screening in those patients whose CSF puncture is counter-indicated.
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Cerebrospinal fluid, Enzyme-linked immunosorbent assay, Neurocysticercosis, Serum, Taenia crassiceps, diagnostic agent, helminth antibody, immunoglobulin G, parasite antigen, animal, blood, case control study, cerebrospinal fluid, comparative study, cross reaction, enzyme linked immunosorbent assay, female, human, immunology, male, neurocysticercosis, Taenia solium, Animals, Antibodies, Helminth, Antigens, Helminth, Case-Control Studies, Cross Reactions, Enzyme-Linked Immunosorbent Assay, Female, Humans, Immunoglobulin G, Male
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Memorias do Instituto Oswaldo Cruz, v. 100, n. 4, p. 427-429, 2005.
