Micropropagación de Pothomorphe umbellata (L.) Miq. vía organogénesis directa
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Data
2007-10-01
Autores
Borda-Yepez, Charlotte Cesty [UNESP]
Tejada, Erik C. Saenz [UNESP]
Lima, Giuseppina Pace Pereira [UNESP]
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Resumo
The present research was aimed at standardizing the protocol of seed disinfection, seed germination and organogenesis via callus of Pothomorphe umbellata (L.) Miq.The germinated seeds were inoculated in different concentrations of BAP (benzylamine purine) and NAA (naphthalene acetic acid) in order to stimulate the callus induction. After 60 days of culture, the calluses with some shoots were taken to an organogenesis medium (GA3 0.1 mg.L -1, BAP 0.5 mg.L-1) during 40 days, to be transferred later to a development medium. Finally, the plantules were acclimatized, presenting a good index of survival.
Descrição
Palavras-chave
Growth regulators, Medicinal plants, Pothomorphe umbellata, Lepianthes peltata
Como citar
Revista Cubana de Plantas Medicinales, v. 12, n. 4, 2007.