Thermal studies on protein isolates of white lupin seeds (Lupinus albus)

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Data

2012-04-01

Autores

Fontanari, Gustavo Guadagnucci [UNESP]
Martins, Jose Manuel
Kobelnik, Marcelo
Pastre, Ieda Aparecida [UNESP]
Gomes Areas, Jose Alfredo
Batistuti, José Paschoal [UNESP]
Fertonani, Fernando Luís [UNESP]

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Editor

Springer

Resumo

This study used TG, DSC, and SDS-PAGE techniques to study protein isolates (PIs) in the powder form obtained from lupin seeds flour Lupinus albus. Different methods of preparing PIs were tested, resulting in final products that were different only in relation to the yield and protein content. The results of the protein analysis by SDS-PAGE showed that the same protein fractions were present in the lupin seeds and in the obtained PIs. This result shows that the process of extraction was not damaging to the composition of the original protein. on the other hand, the results of the thermal analysis (DSC and TG-DTG curves) obtained for the different PIs, led to the detection of changes in the protein conformation through the Delta H values, which in general decreased with increasing values of pH and ionic strength in the experimental conditions of extraction.

Descrição

Palavras-chave

Lupinus albus, TG, DSC, Protein isolate, SDS-PAGE

Como citar

Journal of Thermal Analysis and Calorimetry. Dordrecht: Springer, v. 108, n. 1, p. 141-148, 2012.