Inhibition of peroxidase activity and scavenging of reactive oxygen species by astilbin isolated from Dimorphandra mollis (Fabaceae, Caesalpinioideae)
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Data
2010-01-01
Autores
Petacci, Fernando
Freitas, Silvia S.
Brunetti, Iguatemy Lourenço [UNESP]
Khalil, Najeh M.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Soc Biolgia Chile
Resumo
Astilbin (5,7,3',4'-tetrahydroxy-2,3-dihydroflavonol-3-beta-o-rhamnoside), a flavonoid with a large range of biological activities, was isolated from Dimorphandra molls, a shrub common to the Brazilian Cerrado. The purpose of this study is to verify the effects of astilbin on myeloperoxidase (MPO) and horseradish peroxidase (HRP), and its antioxidant activity against hypochlorous acid (HOCl) and total antioxidant activity (TAC) by the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS center dot+). Astilbin inhibited MPO and HRP activities in a concentration-dependent relationship and effectively scavenged HOC. The TAC by ABTS center dot+ of astilbin (IC50 similar to 20 mM) was higher than that of uric acid, which was used as a positive control. These data demonstrate that astilbin is a potent antioxidant and that it inhibits MPO and HRP activities efficiently.
Descrição
Palavras-chave
Astilbin, Myeloperoxidase, Horseradish peroxidase, Reactive Oxygen Species, Hypochlorous acid
Como citar
Biological Research. Santiago: Soc Biolgia Chile, v. 43, n. 1, p. 63-74, 2010.