Transcription of the Hsp30, Hsp70, and Hsp90 heat shock protein genes is modulated by the PalA protein in response to acid pH-sensing in the fungus Aspergillus nidulans
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Data
2011-09-01
Autores
Freitas, Janana S.
Silva, Emiliana M.
Leal, Juliana
Gras, Diana E.
Martinez-Rossi, Nilce M.
dos Santos, Lucilene Delazari [UNESP]
Palma, Mario Sergio [UNESP]
Rossi, Antonio
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Springer
Resumo
Heat shock proteins are molecular chaperones linked to a myriad of physiological functions in both prokaryotes and eukaryotes. In this study, we show that the Aspergillus nidulans hsp30 (ANID_03555.1), hsp70 (ANID_05129.1), and hsp90 (ANID_08269.1) genes are preferentially expressed in an acidic milieu, whose expression is dependent on the palA (+) background under optimal temperature for fungal growth. Heat shock induction of these three hsp genes showed different patterns in response to extracellular pH changes in the palA(+) background. However, their accumulation upon heating for 2 h was almost unaffected by ambient pH changes in the palA (-) background. The PalA protein is a member of a conserved signaling cascade that is involved in the pH-mediated regulation of gene expression. Moreover, we identified several genes whose expression at pH 5.0 is also dependent on the palA (+) background. These results reveal novel aspects of the heat- and pH-sensing networks of A. nidulans.
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Aspergillus nidulans, pH sensing, pal signaling pathway, HSP
Como citar
Cell Stress & Chaperones. Dordrecht: Springer, v. 16, n. 5, p. 565-572, 2011.