Morphological changes in the fast vs slow fiber profiles of the urethras of diabetic pregnant rats
Nenhuma Miniatura disponível
Data
2011-12-01
Autores
Marini, Gabriela [UNESP]
Barbosa, Angélica M. Pascon [UNESP]
Damasceno, Débora Cristina [UNESP]
Matheus, Selma Maria Michelin [UNESP]
Castro, Rodrigo de Aquino
Girão, Manoel João B. Castello
Rudge, Marilza Vieira Cunha [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Background: This study was undertaken to test the hypothesis that diabetes and pregnancy detrimentally affect the normal function of urethral striated muscles in rats, providing a model for additional studies related to urinary incontinence. The aim of this study was to evaluate morphological alterations in the urethral striated muscles of diabetic pregnant rats. Materials and Methods: Twenty female Wistar rats were distributed into four experimental groups of five rats as follows: virgin, pregnant, diabetic virgin, and diabetic pregnant. Diabetes was induced using streptozotocin administration (40 mg/kg i.v.). The rats were lethally anesthetized, and the urethra and vagina were extracted as a unit. Cryostat sections (6 μm thick) were cut and stained with hematoxylin-eosin, and immunohistochemical procedures were performed and subjected to morphological and semi quantitative analysis. Results: The urethral striated muscle from the diabetic pregnant rats presented with the following variations: thinning and atrophy, disorganization and disruption associated with the colocalization of fast and slow fibers and a steady decrease in the proportion of fast vs slow fibers. Conclusion: Diabetes and pregnancy impair the urethral striated muscle and alter its fiber type distribution. © Copyright G. Marini et al., 2011.
Descrição
Palavras-chave
Diabetes, Pregnancy, Striated muscle fiber, Urethra
Como citar
Urogynaecologia International Journal, v. 25, n. 1, 2011.