Keratinase production by new strain of Bacillus Amyloliquefaciens: Application of statistical experimental design for optimization of keratinase production
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Data
2012-12-17
Autores
Fonseca, Carolina Ferrarezi da [UNESP]
Cortezi, Mariana [UNESP]
Bolner de Lima, Cristian J. [UNESP]
Marchetto, Reinaldo [UNESP]
Macari, Marcos [UNESP]
Contiero, Jonas [UNESP]
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Resumo
Feathers are rich in amino acids and can be employed as a dietary protein supplement for animal feed. Microbial degradation is an alternative technology for improving the nutritional value of feathers. Other potential applications of keratinase include use in the leather industry, detergents and medicine as well as the pharmaceutical for the treatment of acne, psoriasis and calluses. A new keratinolytic enzyme production bacterium was isolated from a poultry processing plant. To improve keratinase yield, statistically based experimental designs were applied to optimize three significant variables: temperature, substrate concentration (feathers) and agitation speed. Response surface methodology demonstrated an increase in keratinolytic activity at temperature, agitation speed and substrate concentration of 26.6°C, 150 rpm and 2%, respectively. Liquid chromatography revealed the release of amino acids in the Bacillus amyloliquefaciens culture broth, thereby demonstrating the potential of feather meal in the animal feed industry. © Global Science Publications.
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Bacillus amyloliquefaciens, Feather degradation, Fermentation, Keratinase, Statistical experimental design, amino acid, keratinase, animal food, bacterial strain, bacterium culture, bacterium isolation, biostatistics, enzyme synthesis, feather, liquid chromatography, nonhuman, poultry, process optimization, temperature, Animalia, Bacteria (microorganisms)
Como citar
Asian Journal of Microbiology, Biotechnology and Environmental Sciences, v. 14, n. 3, p. 307-317, 2012.