A hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells
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Data
2013-01-01
Autores
Cavalcanti, Bruno N. [UNESP]
Zeitlin, Benjamin D.
Nör, Jacques E.
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Resumo
Objectives: The clinical translation of stem cell-based Regenerative Endodontics demands further development of suitable injectable scaffolds. Puramatrix™ is a defined, self-assembling peptide hydrogel which instantaneously polymerizes under normal physiological conditions. Here, we assessed the compatibility of Puramatrix™ with dental pulp stem cell (DPSC) growth and differentiation. Methods: DPSC cells were grown in 0.05-0.25% Puramatrix™. Cell viability was measured colorimetrically using the WST-1 assay. Cell morphology was observed in 3D modeling using confocal microscopy. In addition, we used the human tooth slice model with Puramatrix™ to verify DPSC differentiation into odontoblast-like cells, as measured by expression of DSPP and DMP-1. Results: DPSC survived and proliferated in Puramatrix™ for at least three weeks in culture. Confocal microscopy revealed that cells seeded in Puramatrix™ presented morphological features of healthy cells, and some cells exhibited cytoplasmic elongations. Notably, after 21 days in tooth slices containing Puramatrix™, DPSC cells expressed DMP-1 and DSPP, putative markers of odontoblastic differentiation. Significance: Collectively, these data suggest that self-assembling peptide hydrogels might be useful injectable scaffolds for stem cell-based Regenerative Endodontics. © 2012 Academy of Dental Materials.
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Palavras-chave
Dental pulp, Hydrogel, Odontoblast, Regenerative Endodontics, Stem cells, Tissue engineering, 3-d modeling, Cell morphology, Cell viability, Cell-based, Clinical translation, Human teeths, Hydrogel scaffolds, Injectable scaffolds, Morphological features, Physiological condition, PuraMatrix, Self-assembling peptides, Cell culture, Confocal microscopy, Dentistry, Hydrogels, Scaffolds (biology)
Como citar
Dental Materials, v. 29, n. 1, p. 97-102, 2013.