Publicação: New label free CA125 detection based on gold nanostructured screen-printed electrode
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This paper describes the optimisation and the analytical performances of a label-free impedimetric immunosensor for the detection of tumour marker CA125 based on gold nanoparticles modified screen-printed graphite electrode. Experimental conditions of each step for the developed immunosensor were studied and optimised. The immunosensor response varied linearly (r2 = 0.996) with antigen concentration between 0 and 100 U/mL. The estimated detection limit was 6.7 U/mL. The electrochemical immunosensor allowed unambiguous identification of CA125, while no significant non-specific signal was detected in the case of all negative controls. The analytical usefulness of the impedimetric immunosensor was finally demonstrated analysing serum samples. © 2012 Elsevier B.V. All rights reserved.
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CA125, Gold nanoparticles, Impedimetric immunosensor, Screen-printed electrode, Tumour marker, Analytical performance, Detection limits, Electrochemical immunosensors, Experimental conditions, Gold Nanoparticles, Impedimetric immunosensors, Screen printed electrodes, Gold, Graphite electrodes, Metal nanoparticles, Tumors, Immunosensors
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Sensors and Actuators, B: Chemical, v. 179, p. 194-200.
