Enhanced nicotine-seeking behavior following pre-exposure to repeated cocaine is accompanied by changes in BDNF in the nucleus accumbens of rats
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2013-03-01
Autores
Leão, Rodrigo M. [UNESP]
Cruz, Fábio C. [UNESP]
Carneiro-De-Oliveira, Paulo E. [UNESP]
Rossetto, Daniella B. [UNESP]
Valentini, Sandro Roberto [UNESP]
Zanelli, Cleslei Fernando [UNESP]
Planeta, Cleopatra da Silva [UNESP]
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Resumo
We investigated the behavioral and molecular interactions between cocaine and nicotine, through evaluating locomotor activity, nicotine intravenous self-administration and gene expression. Locomotor sensitization was induced in male Wistar rats by repeated cocaine (20 mg/kg; i.p.) or saline injections once a day over 7 days. Three days after the last injection, rats were challenged with either saline or cocaine (15 mg/kg; i.p.) and the locomotor activity was measured. The very next day animals received either saline or nicotine (0.4 mg/kg; s.c.) and the locomotor cross-sensitization was tested. Animals were then prepared with intrajugular catheters for nicotine self-administration. Nicotine self-administration patterns were evaluated using fixed or progressive ratio schedules of reinforcement and a 24-h unlimited access binge. Immediately after the binge sessions animals were decapitated, the brains were removed and the nucleus accumbens was dissected. The dynorphin (DYN), μ-opioid receptor (mu opioid), neuropeptide Y (NPY), brain-derived neurotrophic factor (BDNF), tropomyosin-related tyrosine kinase B receptor (TrkB) and corticotropin- releasing factor receptor type 1 (CRF-R1) gene expression were measured by the reverse transcription-polymerase chain reaction (RT-PCR). Pretreatment with cocaine caused sensitization of cocaine motor response and locomotor cross-sensitization with nicotine. In the self-administration experiments repeated cocaine administration caused an increase in the nicotine break point and nicotine intake during a 24 h binge session. © 2013 Elsevier Inc.
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Palavras-chave
BDNF, Cocaine, Gene expression, Locomotor activity, Nicotine, RT-PCR, Self-administration, brain derived neurotrophic factor, brain derived neurotrophic factor receptor, cocaine, corticotropin releasing factor, dynorphin, mu opiate receptor, nicotine, sodium chloride, animal experiment, animal model, animal tissue, brain tissue, controlled study, cross allergy, decapitation, drug seeking behavior, gene expression, locomotion, male, nonhuman, nucleus accumbens, priority journal, rat, reinforcement, reverse transcription polymerase chain reaction, Animals, Brain-Derived Neurotrophic Factor, Dynorphins, Gene Expression, Male, Motor Activity, Neuropeptide Y, Nucleus Accumbens, Rats, Rats, Wistar, Receptor, trkB, Receptors, Corticotropin-Releasing Hormone, Receptors, Opioid, mu, Risk Factors, Self Administration, Tobacco Use Disorder, Animalia, Rattus, Rattus norvegicus
Como citar
Pharmacology Biochemistry and Behavior, v. 104, n. 1, p. 169-176, 2013.