Publicação: Immunosensor based on immobilization of antigenic peptide NS5A-1 from HCV and silk fibroin in nanostructured films
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Data
2013-03-19
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Coorientador
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Resumo
The peptide NS5A-1 (PPLLESWKDPDYVPPWHG), derived from hepatitis C virus (HCV) NS5A protein, was immobilized into layer-by-layer (LbL) silk fibroin (SF) films. Deposition was monitored by UV-vis absorption measurements at each bilayer deposited. The interaction SF/peptide film induced secondary structure in NS5A-1 as indicated by fluorescence and circular dichroism (CD) measurements. Voltammetric sensor (SF/NS5A-1) properties were observed when the composite film was tested in the presence of anti-HCV. The peptide-silk fibroin interaction studied here showed new architectures for immunosensors based on antigenic peptides and SF as a suitable immobilization matrix. © 2013 American Chemical Society.
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Antigenic peptides, Hepatitis C virus, Immobilization matrices, Layer-by-layers, Nanostructured Films, Secondary structures, UV-vis absorptions, Voltammetric sensor, Antigens, Composite films, Immunosensors, Viruses, Peptides, antigen, fibroin, immobilized protein, nanomaterial, NS 5 protein, hepatitis C virus, NS-5 protein, hepatitis C virus, peptide fragment, virus protein, amino acid sequence, animal, chemistry, genetic procedures, immunology, methodology, molecular genetics, Amino Acid Sequence, Animals, Biosensing Techniques, Fibroins, Immobilized Proteins, Molecular Sequence Data, Nanostructures, Peptide Fragments, Viral Nonstructural Proteins
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Inglês
Como citar
Langmuir, v. 29, n. 11, p. 3829-3834, 2013.
