Publication: Topical application of the lectin Artin M accelerates wound healing in rat oral mucosa by enhancing TGF-β and VEGF production
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Date
2013-05-01
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Coadvisor
Graduate program
Undergraduate course
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Article
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Abstract
The lectin Artin M has been shown to accelerate the wound-healing process. The aims of this study were to evaluate the effects of Artin M on wound healing in the palatal mucosa of rats and to investigate the effects of Artin M on transforming growth factor beta (TGF-β) and vascular endothelial growth factor (VEGF) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment: C - Control (nontreated), A - Artin M gel, and V - Vehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5 μg/mL of Artin M for 24, 48, and 72 hours. The expression of VEGF and TGF-β was determined by enzyme-linked immunosorbent assay. Histologically, at day 7, the Artin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups (p < 0.05). Artin M-induced cell proliferation in vivo and promoted a greater expression of TGF-β and VEGF in both experiments (p < 0.05). Artin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF-β and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers. © 2013 by the Wound Healing Society.
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Keywords
collagen fiber, interleukin 1, interleukin 6, lectin, transforming growth factor beta, vasculotropin, animal cell, animal tissue, cell proliferation, cheek mucosa, collagen synthesis, controlled study, enzyme linked immunosorbent assay, epithelization, fibroblast culture, gel, histology, immunohistochemistry, in vivo study, male, mouth lesion, nonhuman, priority journal, protein expression, rat, secretion (process), surgical wound, wound healing
Language
English
Citation
Wound Repair and Regeneration, v. 21, n. 3, p. 456-463, 2013.
