Gastroprotective mechanisms of the chloroform and ethyl acetate phases of Praxelis clematidea (Griseb.) R.M.King & H.Robinson (Asteraceae)
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2013-07-01
Autores
Falcão, Heloina de Sousa
Maia, Gabriela Lemos de Azevedo
Bonamin, Flávia [UNESP]
Kushima, Hélio [UNESP]
Moraes, Thiago Mello [UNESP]
Hiruma Lima, Clélia Akiko [UNESP]
Takayama, Christiane
Ferreira, Anderson Luiz
Souza Brito, Alba Regina Monteiro
Agra, Maria de Fátima
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Resumo
Flavonoid-rich Praxelis clematidea (Griseb.) R.M.King & H.Robinson (Asteraceae) is a native plant of South America. This study evaluates the gastroprotective activity and possible mechanisms for both the chloroform (CHCl3P) and ethyl acetate phases (AcOEtP) obtained from aerial parts of the plant. The activity was investigated using acute models of gastric ulcer. Gastric secretion biochemical parameters were determined after pylorus ligature. The participation of cytoprotective factors such as mucus, nitric oxide (NO), sulfhydryl (SH) groups, prostaglandin E2 (PGE 2), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), reduction of lipid peroxidation (malondialdehyde level), and polymorphonuclear infiltration (myeloperoxidase activity), was also investigated. CHCl3P (125, 250, and 500 mg/kg) and AcOEtP (62.5, 125, and 250 mg/kg) showed significant gastroprotective activity, reducing the ulcerative index by 75, 83, 88 % and 66, 66, 81 % for ethanol; 67, 67, 56 % and 56, 53, 58 % for a non-steroidal anti-inflammatory drug (NSAID); and 74, 58, 59 % and 64, 65, 61 % for stress-induced gastric ulcer, respectively. CHCl3P (125 mg/kg) and AcOEtP (62.5 mg/kg) significantly reduced the ulcerative area by 78 and 83 %, respectively, for the ischemia-reperfusion model. They also did not alter the biochemical parameters of gastric secretion, the GSH level or the activities of SOD, GPx or GR. They increased the quantity of gastric mucus, not dependent on NO, yet dependent on SH groups, and maintained PGE2 levels. The P. clematidea phases demonstrated gastroprotective activity related to cytoprotective factors. © 2012 The Japanese Society of Pharmacognosy and Springer.
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Asteraceae, Cytoprotection, Gastric ulcer, Gastroprotective activity, Praxelis clematidea, 7,4' dimethylapigenin, acetic acid ethyl ester, apigenin, chloroform, cimetidine, cirsimaritin, gastrointestinal mucosa protective agent, genkwanin, glutathione, glutathione peroxidase, glutathione reductase, lansoprazole, malonaldehyde, myeloperoxidase, nitric oxide, plant extract, plant medicinal product, Praxelis clematidea extract, prostaglandin E2, superoxide dismutase, tetramethylscutellarein, thiol group, trimethylapigenin, unclassified drug, animal experiment, animal model, animal tissue, antioxidant activity, cell infiltration, controlled study, drug identification, drug isolation, drug mechanism, drug structure, enzyme activity, lipid peroxidation, male, mouse, nonhuman, polymorphonuclear cell, single drug dose, stomach mucus, stomach protection, stomach secretion, stomach ulcer
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Journal of Natural Medicines, v. 67, n. 3, p. 480-491, 2013.