Association of the Leptin Gene with Carcass Characteristics in Nellore Cattle
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Data
2013-07-08
Autores
de Oliveira, Joyce Azambuja
Cunha, Camila Magalhães da
Crispim, Bruno do Amaral
Seno, Leonardo de Oliveira
Fernandes, Alexandre Rodrigo Mendes
Nogueira, Guilherme de Paula [UNESP]
Grisolia, Alexéia Barufatti
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Resumo
Advances in DNA technology have created biotechnological tools that can be used in animal selection and new strategies for increasing herd productivity and quality. The objective of the present work was to associate the genotypes of leptin gene exon 2 polymorphisms with productive traits in Nellore cattle. Blood was collected from Nellore males and PCR-RFLP reactions were performed with the restriction enzymes ClaI and Kpn2I. The gene frequencies resulting from digestion by ClaI were 0.60 and 0.40 for allele A and T, respectively; the genotypic frequencies were AA = 0.20 and AT = 0.80. The gene frequencies from digestion by Kpn2I were 0.81 for allele C and 0.194 for allele T; the genotypic frequencies were CC = 0.62 and CT = 0.38. The populations in both cases were not in Hardy-Weinberg equilibrium (p > 0.05), and the TT genotype was not found. Significant associations were noted between leptin gene exon 2 polymorphisms and five productive traits in Nellore cattle: carcass fat distribution, the intensity of red muscle coloration, pH, marbling, and post-slaughter fat thickness. © 2013 Copyright Taylor and Francis Group, LLC.
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Animal selection, Bos indicus, Genetic improvement, PCR-RFLP, Biotechnological tools, DNA technology, Gene frequency, Genetic improvements, Hardy-Weinberg equilibrium, Restriction enzymes, Animals, Hormones, Polymerase chain reaction, Genes
Como citar
Animal Biotechnology, v. 24, n. 3, p. 229-242, 2013.