A PCR/RFLP methodology to identify non-Amazonian Brazilian deer species
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Data
2013-09-01
Autores
de Souza, Juliana Nogueira [UNESP]
de Oliveira, Márcio Leite [UNESP]
Duarte, José Maurício Barbanti [UNESP]
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Resumo
Due to the necessity of using noninvasive samples to study animals as elusive as the deer that occur in Brazil, we realized it was important to develop a PCR/RFLP protocol to assist in identifying such samples. Thus we developed a protocol in which a fragment of the cytochrome b gene is digested with two enzymes: SspI, which distinguishes species of the genus Mazama from Blastocerus dichotomus and Ozotoceros bezoarticus, and TAQα1 which permits differentiation between the species B. dichotomus and O. bezoarticus. © 2013 Springer Science+Business Media Dordrecht.
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Blastocerus, Cervidae, Mazama, Ozotoceros, Restriction enzymes
Como citar
Conservation Genetics Resources, v. 5, n. 3, p. 639-641, 2013.