Effect of the isocoumarin paepalantine on the luminol and lucigenin amplified chemiluminescence of rat neutrophils
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Data
2003-06-01
Autores
Kitagawa, R. R.
Raddi, MSG
Khalil, N. M.
Vilegas, Wagner [UNESP]
da Fonseca, L. M.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Pharmaceutical Soc Japan
Resumo
Paepalantine (9,10-dihydroxy-5,7-dimethoxy-1H-naphto(2,3c)pyran-1-one), a natural isocoumarin isolated from the capitula of Paepalanthus bromelioides (Eriocaulaceae), was assessed for its effect on the respiratory burst (zymosan-stimulated luminol-enhanced chemiluminescence and. PMA-stimulated lucigenin-enhanced chemiluminescence) of polymorphonuclear neutrophils in vitro. Special attention was devoted to establishing the IC50 for neutrophils. Paepalantine was able to decrease luminol and lucigenin chemiluminescence, reflecting an inhibitory effect on the respiratory burst, with an ED50 of 0.44 +/- 0.05 and 0.84 +/- 0.15 mug/ml, respectively. A cell-free system was performed with paepalantine on myeloperoxidase/H2O2 and myeloperoxidase/H2O2/Cl- systems. Paepalantine inhibited luminol oxidation in both systems. This inhibition was related to the interaction of paepalantine-myeloperoxidase and its scavenger effect on HOCl.
Descrição
Palavras-chave
antioxidant activity, paepalantine, isocoumarin, chemiluminescence
Como citar
Biological & Pharmaceutical Bulletin. Tokyo: Pharmaceutical Soc Japan, v. 26, n. 6, p. 905-908, 2003.