Publicação: Karyotypic conservatism in five species of Prochilodus (Characiformes, Prochilodontidae) disclosed by cytogenetic markers
Carregando...
Arquivos
Data
2013-09-05
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Tipo
Artigo
Direito de acesso
Acesso aberto
Resumo
The family Prochilodontidae is considered a group with well conserved chromosomes characterized by their number, morphology and banding patterns. Thence, our study aimed at accomplishing a cytogenetic analysis with conventional methods (Giemsa staining, silver staining of the nucleolus organizer regions-AgNOR, and C-banding) and fluorescence in situ hybridization (FISH) with 18S and 5S ribosomal DNA probes in five species of the Prochilodus genus (Prochilodus argenteus, Prochilodus brevis, Prochilodus costatus, Prochilodus lineatus and Prochilodus nigricans) collected from different Brazilian hydrographic basins. The results revealed conservatism in chromosome number, morphology, AgNORs 18S and 5S rDNAs location and constitutive heterochromatin distribution patterns. The minor differences observed in this work, such as an Ag-NOR on a P. argenteus chromosome and a distinct C-banding pattern in P. lineatus, are not sufficient to question the conservatism described for this group. Future work using repetitive DNA sequences as probes for FISH will be interesting to further test the cytogenetic conservatism in Prochilodus. © 2013, Sociedade Brasileira de Genética.
Descrição
Palavras-chave
AgNOR, C-banding, Conserved karyotype, FISH, Hydrographic basins, DNA 18S, DNA 5S, C banding, Characiformes, chromosome marker, chromosome NOR, chromosome number, chromosome structure, cytogenetics, DNA probe, fluorescence in situ hybridization, genetic conservation, Giemsa stain, heterochromatin, hydrography, karyotype, nonhuman, Prochilodus, Prochilodus argenteus, Prochilodus brevis, Prochilodus costatus, Prochilodus lineatus, Prochilodus nigricans, river basin, silver staining
Idioma
Inglês
Como citar
Genetics and Molecular Biology, v. 36, n. 3, p. 347-352, 2013.
