Carbon fiber microelectrodes for adsorptive stripping analysis of trace nucleic acids
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Elsevier B.V.
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Artigo
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Resumo
Carbon fiber ultramicroelectrodes are shown to be suitable for adsorptive stripping potentiometric measurements of trace DNA and RNA. The origin of the carbon fiber has a profound effect upon its suitability for trace analysis of nucleic acids, with the 'Aesar' materials performing most favorably. The resulting ultramicroelectrodes offer effective adsorptive accumulation of DNA and RNA from unstirred microliter-volume solutions, and are shown to be useful in adsorptive stripping transfer experiments. The influence of the surface pretreatment and accumulation conditions is described, along with the analytical-performance characteristics. The detection limits are 6, 15 and 40 mu g/l tRNA, ssDNA and dsDNA, respectively (5 min accumulation). (C) 1998 Elsevier B.V. S.A.
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nucleic acids, DNA, RNA, carbon fiber electrode, microelectrode, chronopotentiometry, potentiometric stripping analysis
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Inglês
Citação
Journal of Electroanalytical Chemistry. Lausanne: Elsevier B.V. Sa, v. 441, n. 1-2, p. 167-172, 1998.
