Estudo comparativo de reparação óssea em ratas ovariectomizadas tratadas com risedronato e Calcarea fluorica
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Data
2006-07-17
Autores
Senra, Giselle Segnini [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O aumento da expectativa de vida amplia as possibilidades de doenças degenerativas, como osteopenia e osteoporose, que elevam a ocorrência de fraturas e demandam tratamento prolongado. Os medicamentos usados no tratamento da osteoporose podem interferir no reparo ósseo de fraturas. O objetivo deste trabalho foi avaliar o efeito do Risedronato (1,5mg/kg/dia) e do medicamento homeopático Calcarea fluorica 6CH (3 gotas/dia) no reparo ósseo em ratas com osteopenia induzida. Para isso, 105 ratas foram submetidas à ovariectomia e 35 ratas à cirurgia sham. Através da histomorfometria, foi avaliada a porcentagem de preenchimento com osso neoformado na área total disponível compreendendo o defeito e a cavidade medular e também a porcentagem de osso trabecular dentro da área preenchida pelo calo ósseo aos seis, 12, 18 e 24 dias de tratamento (n=6). Em relação à área de preenchimento total, o grupo R superou os valores dos demais (p<0,05) enquanto que os grupos Cf e O se mostraram com valores iguais em todos os períodos de tempo, diferindo somente aos seis dias, quando o grupo Cf foi superior ao O. A porcentagem de área trabecular dentro da área preenchida pelo calo aos seis, 12 e 18 dias não foi diferente entre os grupos R, Cf e O. Aos 24 dias o grupo O apresentou os maiores valores de área trabecular, sendo diferente do grupo R, com os menores valores, e o grupo Cf mostrou valores intermediários. Concluiu-se que a ovariectomia interferiu de forma prejudicial no reparo e que o medicamento risedronato foi o que estimulou a maior formação óssea e a menor remodelação. A Calcarea fluorica 6CH estimulou maior formação óssea nas fases iniciais da reparação quando comparada ao grupo O, mas não interferiu negativamente na remodelação.
The increase in life expectancy is associated with a higher incidence of degenerative diseases such as osteopenia and osteoporosis, which elevate the occurrence of bone fractures, and demand long treatment. The medication used to treat osteoporosis can interfere with bone fractures repair. The aim of this work was evaluating the effects of Risedronate (1,5mg/Kg/day) and of the homeopathic medicine Calcarea fluorica 6CH (3 drops/day) on bone repair in rats with induced osteopenia. For that, 105 rats were ovariectomized and 35 were sham operated. After 35 days, 2,5 mm monocortical bone lesions were drilled on all animals tibiae. Then, treatment began. Data were submitted to ANOVA and Tukey (5%) tests. According to optical density analysis, there were no differences between treatments at three, six and 12 days, but at 18 and 24 days, group R showed the highest values (p<0,05). Histomorphometric analysis evaluated the filling percentage by newly formed bone within the total area available in the defect and medullar cavity together; and also the percentage of trabecular bone within the callus. According to the first measure, the group R showed the highest values in all periods, surpassing all the groups (p<0,05) while the groups Cf and O showed similar results in all periods, except on day 6, when group Cf was higher. The measure of the trabecular area in the callus showed no difference between the groups R, Cf and O on the 6th, 12th and 18th days. On the 24th day, group O showed the higher trabecular value and group R showed the lower value. It was possible to conclude that ovariectomy has interfered negatively on the bone repair process and that Risedronate stimulated a great amount of bone formation with low bone turn over. Calcarea fluorica 6CH stimulated high bone formation at the initial stages of bone repair when compared to the control group but didn't inhibit bone remodelation.
The increase in life expectancy is associated with a higher incidence of degenerative diseases such as osteopenia and osteoporosis, which elevate the occurrence of bone fractures, and demand long treatment. The medication used to treat osteoporosis can interfere with bone fractures repair. The aim of this work was evaluating the effects of Risedronate (1,5mg/Kg/day) and of the homeopathic medicine Calcarea fluorica 6CH (3 drops/day) on bone repair in rats with induced osteopenia. For that, 105 rats were ovariectomized and 35 were sham operated. After 35 days, 2,5 mm monocortical bone lesions were drilled on all animals tibiae. Then, treatment began. Data were submitted to ANOVA and Tukey (5%) tests. According to optical density analysis, there were no differences between treatments at three, six and 12 days, but at 18 and 24 days, group R showed the highest values (p<0,05). Histomorphometric analysis evaluated the filling percentage by newly formed bone within the total area available in the defect and medullar cavity together; and also the percentage of trabecular bone within the callus. According to the first measure, the group R showed the highest values in all periods, surpassing all the groups (p<0,05) while the groups Cf and O showed similar results in all periods, except on day 6, when group Cf was higher. The measure of the trabecular area in the callus showed no difference between the groups R, Cf and O on the 6th, 12th and 18th days. On the 24th day, group O showed the higher trabecular value and group R showed the lower value. It was possible to conclude that ovariectomy has interfered negatively on the bone repair process and that Risedronate stimulated a great amount of bone formation with low bone turn over. Calcarea fluorica 6CH stimulated high bone formation at the initial stages of bone repair when compared to the control group but didn't inhibit bone remodelation.
Descrição
Palavras-chave
Homeopatia, Osteoporose, Dentes - Radiografia, Difosfonatos, Radiografia dentária digital, Histomorfometria, Osteoporosis, Calcarea fluorica, Homeopathy, Allopathy, Histomorphometry
Como citar
SENRA, Giselle Segnini. Estudo comparativo de reparação óssea em ratas ovariectomizadas tratadas com risedronato e Calcarea fluorica. 2006. 145 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Odontologia de São José dos Campos, 2006.