Transcriptoma (RNA-seq) de laranja doce Valência (Citrus sinensis L. Osbeck) e Kumquat (Fortunella spp.) infectadas por Xanthomonas citri subsp. citri
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2013-08-21
Autores
Cofre, Teresa Del Carmen Gabriel [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Dentre as diversas doenças que ocorrem na citricultura, o cancro cítrico é uma das principais, considerada economicamente importante e com impactos diretos e indiretos no setor citrícola mundial. O agente causal do cancro cítrico é a bactéria Xanthomonas citri subsp. citri (Xac), a qual afeta uma ampla gama de espécies e variedades de citros. A variedade de laranja doce Valência (Citrus sinensis) é considerada moderadamente suscetível ao cancro cítrico e a Rutaceae Kumquat (Fortunella spp.), por outro lado, é considerada altamente resistente. Objetivando gerar informações acerca dos mecanismos moleculares que resultam em diferenças quanto à suscetibilidade de plantas a patógenos, no presente estudo fez-se uso de uma nova plataforma de sequenciamento de transcriptoma (RNA-seq) para análise da expressão gênica de Valência e Kumquat após infecção por Xac nos tempos de 24, 48 e 72 horas. Foram identificados 2.953 e 4.111 transcritos induzidos em Valência e Kumquat, respectivamente. Dos transcritos reprimidos Kumquat apresentou 11.041 e Valência 1.930. Na comparação da expressão gênica temporal, Kumquat apresentou muitos transcritos induzidos em comum em todos os tempos de infecção, alguns deles relacionados à percepção de sinais (receptores do tipo quinase) e também envolvidos na defesa de plantas [(como proteínas relacionadas à patogênese (PRs)]. Foram identificados exclusivamente em Kumquat transcritos que codificam para a proteína germin, possivelmente envolvida na resposta de defesa à Xac. Nas comparações entre espécies, em Kumquat observou-se alta expressão de proteínas relacionadas à síntese de metabólitos secundários, como alcaloides e monoterpenos. Em Valência, nos tempos iniciais de 24 e 48 horas após a inoculação de Xac, observou-se alta expressão de cisteína proteinases, as quais comumente...
Among the various diseases that occur in Citrus spp., citrus canker is a serious disease, considered economically important with direct impact on the citrus sector. The causal agent of citrus canker is the bacterium Xanthomonas citri subsp. citri (Xac), and it affects a wide range of Citrus spp.. Sweet orange Valencia Citrus sinensis is considered moderately susceptible to Citrus canker and the Rutaceae Kumquat Fortunella spp. is considered highly resistant. To understand the molecular mechanisms that distinguish plant resistance in plant pathogen interaction, the present study used a new transcriptome sequencing platform (RNA-seq) to analyze gene expression of Valencia and Kumquat after infection by Xac post 24, 48 and 72 hours. We identified 2,953 and 4,111 transcripts induced in Valencia and Kumquat, respectively. Among repressed transcripts Kumquat showed 11,041 and Valencia 1,930. In the comparison of temporal expression between the evaluated times, Kumquat presented many transcripts induced in common at all times of infection that are related to signal perception, as receptors kinase and plant defense proteins as pathogenesis related proteins (PRs). Were found exclusively in Kumquat transcripts that encode for protein Germin possibly involved in defense response to Xac. In the analysis between species, for Kumquat were found a high expression of proteins related to the synthesis of secondary metabolites, such as alkaloids and monoterpenes. These products may be mediated by biotic factors such as pathogen attack, so the high expression of genes related to metabolic production of these can be related to plant defense. Valencia presented at 24 and 48 hours high expression of cysteine proteinases. These are involved in reconstruction and degradation of proteins in response to different stimuli and also... (Complete abstract click electronic access below)
Among the various diseases that occur in Citrus spp., citrus canker is a serious disease, considered economically important with direct impact on the citrus sector. The causal agent of citrus canker is the bacterium Xanthomonas citri subsp. citri (Xac), and it affects a wide range of Citrus spp.. Sweet orange Valencia Citrus sinensis is considered moderately susceptible to Citrus canker and the Rutaceae Kumquat Fortunella spp. is considered highly resistant. To understand the molecular mechanisms that distinguish plant resistance in plant pathogen interaction, the present study used a new transcriptome sequencing platform (RNA-seq) to analyze gene expression of Valencia and Kumquat after infection by Xac post 24, 48 and 72 hours. We identified 2,953 and 4,111 transcripts induced in Valencia and Kumquat, respectively. Among repressed transcripts Kumquat showed 11,041 and Valencia 1,930. In the comparison of temporal expression between the evaluated times, Kumquat presented many transcripts induced in common at all times of infection that are related to signal perception, as receptors kinase and plant defense proteins as pathogenesis related proteins (PRs). Were found exclusively in Kumquat transcripts that encode for protein Germin possibly involved in defense response to Xac. In the analysis between species, for Kumquat were found a high expression of proteins related to the synthesis of secondary metabolites, such as alkaloids and monoterpenes. These products may be mediated by biotic factors such as pathogen attack, so the high expression of genes related to metabolic production of these can be related to plant defense. Valencia presented at 24 and 48 hours high expression of cysteine proteinases. These are involved in reconstruction and degradation of proteins in response to different stimuli and also... (Complete abstract click electronic access below)
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Cancro citrico, Xanthomonas campestris, Expressão gênica, Frutas citricas - Cultivo, Laranja - Doenças e pragas, Citrus fruits
Como citar
COFRE, Teresa Del Carmen Gabriel. Transcriptoma (RNA-seq) de laranja doce Valência (Citrus sinensis L. Osbeck) e Kumquat (Fortunella spp.) infectadas por Xanthomonas citri subsp. citri. 2013. 148 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Farmacêuticas de Araraquara, 2013.