Resposta sorológica em bovinos vacinados contra o carbúnculo sintomático
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Data
2005-08-05
Autores
Lopes, Bruna Pereira [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O Clostridium chauvoei é o agente causador do carbúnculo sintomático, doença que acomete bovinos e ovinos. É um dos principais problemas sanitários que ocorre nos rebanhos pecuários em todo o mundo. A vacinação dos animais jovens é a medida profilática indicada, visto que esta medida preventiva nos sistemas de produção é de extrema importância econômica e reconhecida pelos produtores, mesmo sendo de aplicação voluntária. Três grupos de bovinos, contendo 20 animais em cada grupo, foram vacinados com três vacinas comerciais contra o carbúnculo sintomático para a avaliação sorológica dos mesmos por meio do teste de aglutinação rápida em placa. Os animais foram vacinados no dia 0, e o reforço vacinal foi realizado 30 dias após a primeira dose. Após 21 dias do reforço, o sangue foi coletado da veia jugular, e o soro foi obtido a temperatura ambiente quando ocorreu a coagulação sanguínea e separação do soro. Foi comparada a resposta de indução de anticorpos nos grupos de animais reagentes a esse teste com antígenos obtidos de duas cepas, uma de referência (MT) do Ministério da Agricultura, Pecuária e Abastecimento (MAPA) e outra cepa de campo. Os resultados demonstraram que a resposta dos animais imunizados frente à cepa de referência foi satisfatória, não existindo diferença entre as três vacinas testadas. No entanto, quando este mesmo teste foi realizado com antígenos produzidos com a cepa de campo identificada por PCR, verificou-se a ausência de anticorpos aglutinantes na maioria dos bovinos avaliados. Na análise estatística ficou comprovado que existem diferenças significativas nos resultados dos testes de aglutinação rápida em placa quando se utilizam cepas distintas de Clostridium chauvoei, provavelmente em decorrência de diferenças antigênicas existentes entre as cepas.
The Clostridium chauvoei is the agent which causes black leg, a disease that attacks the bovine and ovine. It is one of the main sanitary problems that occur with cattle-raising flocks in the world. The vaccination of young animals is the best prevention measure in production systems and is of extreme economic importance and is recognized by producers even if the application is voluntary. Three bovine groups, containing 20 animals each group, were vaccinated using three commercial vaccines against the black leg, for evaluation of bovine serological by tests of plate agglutination. The cattle were treated on day 0 and another dose was given on day 30. After 21 days, the blood was colleted of jugular vein and serum was obtained at 25°C, by blood coagulation. The comparison was made by detection of antibodies response between the animals that react to the test with antigens obtained from two strains which were used, one referred (MT) as Ministério da Agricultura, Pecuária e Abastecimento (MAPA) and another strain isolated from the field cases. The results determined that the response of the immunized animals faced by the referred strain was satisfactory with non-existing differences between the three tested vaccines. However, when the same test was conducted with antigens obtained from the field strain identified by PCR, it was verified the absence of agglutinating antibodies agglutinants in the majority of the bovines evaluated. In the statistics analysis were confirmed that significant differences were obtained in the agglutination plate test when different strains of Clostridium chauvoei were used, maybe because of the occurrence of antigenic difference, between the two strains tested.
The Clostridium chauvoei is the agent which causes black leg, a disease that attacks the bovine and ovine. It is one of the main sanitary problems that occur with cattle-raising flocks in the world. The vaccination of young animals is the best prevention measure in production systems and is of extreme economic importance and is recognized by producers even if the application is voluntary. Three bovine groups, containing 20 animals each group, were vaccinated using three commercial vaccines against the black leg, for evaluation of bovine serological by tests of plate agglutination. The cattle were treated on day 0 and another dose was given on day 30. After 21 days, the blood was colleted of jugular vein and serum was obtained at 25°C, by blood coagulation. The comparison was made by detection of antibodies response between the animals that react to the test with antigens obtained from two strains which were used, one referred (MT) as Ministério da Agricultura, Pecuária e Abastecimento (MAPA) and another strain isolated from the field cases. The results determined that the response of the immunized animals faced by the referred strain was satisfactory with non-existing differences between the three tested vaccines. However, when the same test was conducted with antigens obtained from the field strain identified by PCR, it was verified the absence of agglutinating antibodies agglutinants in the majority of the bovines evaluated. In the statistics analysis were confirmed that significant differences were obtained in the agglutination plate test when different strains of Clostridium chauvoei were used, maybe because of the occurrence of antigenic difference, between the two strains tested.
Descrição
Palavras-chave
Vacinas, Bovino, Carbunculo, Soroaglutinação, Agglutination, Black leg, Bovine, Clostridium chauvoei, Vaccines
Como citar
LOPES, Bruna Pereira. Resposta sorológica em bovinos vacinados contra o carbúnculo sintomático. 2005. iv, 50 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, 2005.