Avaliação do método de sucção de encéfalo de morcegos (Chiroptera, Mammalia) para diagnóstico da raiva
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Data
2008-09-26
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Universidade Estadual Paulista (Unesp)
Resumo
O diagnóstico laboratorial da raiva em morcegos é realizado utilizando-se, principalmente, o encéfalo do animal suspeito. O objetivo do presente trabalho foi testar a hipótese de que o método de aspiração com pipeta plástica (tipo Pasteur) é eficaz na obtenção de encéfalo de morcegos para a realização do diagnóstico comparado com o método tradicional de abertura de crânio. Para tanto, estudaram-se quatro espécies diferentes de morcegos, Molossus rufus (E. Geoffroy, 1805), Molossus molossus (Pallas, 1766), Artibeus lituratus (Olfers,1818) e Myotis nigricans (Schinz, 1821), totalizando 200 amostras. A quantidade de massa encefálica obtida pelo método tradicional foi significativamente maior, contudo, o material colhido pela aspiração foi suficiente para a execução do diagnóstico da doença. Ambos os métodos detectaram indivíduos positivos, sendo que o método de aspiração teve a vantagem de preservar o crânio possibilitando melhor identificação das espécies.
Rabies diagnosis in bats is usually performed using the brain of suspected animals. The main hypothesis tested by this work was that aspiration method using plastic pipette (Pasteur type) was effective in the collection of bat brain sample for rabies diagnosis when compared to the opening skull method. A total of two hundred bats of four species were studied: Molossus rufus (E. Geoffroy, 1805), Molossus molossus (Pallas, 1766), Artibeus lituratus (Olfers, 1818) and Myotis nigricans (Schinz, 1821). The proportion of brain weight compared to body weight was statistically higher when using the traditional method, although the brain mass collected by aspiration method was enough for rabies diagnosis. Results demonstrate that independently of the collecting method, both gated to detect positive samples and the aspiration method has the advantage of skull preservation, permitting the identification of the species.
Rabies diagnosis in bats is usually performed using the brain of suspected animals. The main hypothesis tested by this work was that aspiration method using plastic pipette (Pasteur type) was effective in the collection of bat brain sample for rabies diagnosis when compared to the opening skull method. A total of two hundred bats of four species were studied: Molossus rufus (E. Geoffroy, 1805), Molossus molossus (Pallas, 1766), Artibeus lituratus (Olfers, 1818) and Myotis nigricans (Schinz, 1821). The proportion of brain weight compared to body weight was statistically higher when using the traditional method, although the brain mass collected by aspiration method was enough for rabies diagnosis. Results demonstrate that independently of the collecting method, both gated to detect positive samples and the aspiration method has the advantage of skull preservation, permitting the identification of the species.
Descrição
Palavras-chave
Cérebro - Amostragem, Cérebro - Sucção, Cranio, Morcego, Brain - Sampling studies, Brain - Suction, Skull, Chiroptera, Laboratory techniques and procedures
Como citar
GONÇALES, Juliano Ferreira. Avaliação do método de sucção de encéfalo de morcegos (Chiroptera, Mammalia) para diagnóstico da raiva. 2008. 37 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araçatuba, 2008.