Otimização das condições de cultivo do fungo Aspergillus nidulans para produção de melanina usando resíduos agroindustriais
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Data
2009-07-28
Autores
Pretti, Taís Simone [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Devido ao crescente interesse na produção de substâncias ativas a partir de fontes naturais, os fungos vêm sendo considerados um grupo importante de microrganismos em função do seu enorme potencial de exploração, representando uma fonte inesgotável para a obtenção de vários produtos biotecnológicos industriais. Dentre estes produtos, o pigmento melanina produzido pelo fungo Aspergillus nidulans tem considerável potencial biotecnológico para ser usado em formulações cosméticas, principalmente pelas suas atividades antioxidante, antiinflamatória e fotoprotetora. No entanto, para uma possível aplicação prática desta substância é necessário estabelecer as condições ótimas de cultivo do fungo para a produção em larga escala com um custo menor comparado à melanina sintética. Neste contexto, o objetivo deste trabalho foi avaliar a influência do volume de meio de cultura, do tempo de incubação e da utilização de resíduos industriais (água de maceração de milho, bagaço de cana de açúcar e melaço) como fontes de nutrientes no crescimento e produção de pigmento pelas linhagens MEL1 e MEL2 de Aspergillus nidulans. Os resultados mostraram que na presença de 2% de água de maceração de milho o crescimento celular foi maior enquanto que na concentração de 0,2% houve um aumento na produção de pigmento pelas linhagens em estudo. Quando melaço (1% e 10%) foi usado como fonte de carbono, a produção de pigmento foi inibida em ambas as concentrações, enquanto um alto rendimento de biomassa foi obtido com 10% de melaço. A suplementação do meio com hidrolisado do bagaço da cana-de-açúcar não teve um efeito positivo na produção de pigmento, embora tenha proporcionado um aumento no crescimento das linhagens. Também foi observado que a morfologia do fungo (tamanho do “pellet”) influencia a produção de pigmento, sendo que na cultura...
Due to an increasing interest in the production of actives substances from natural sources, fungi has been considered an important group of microorganisms in function of its enormous potential for exploitation, representing an inexhaustible source to obtain several industrials biotechnology products. Among these products, the melanin pigment produced by fungi Aspergillus nidulans has considerable biotechnological potential to be used in cosmetic formulations, mainly for its antioxidant, anti-inflammatory and photoprotetor activities. However, for a possible practical application of this substance, is necessary to establish the optimal conditions of the fungal culture for large scale production with low cost compared to synthetic melanin. In this context, the objective of this work was to evaluate the influence of volume of culture medium, period of incubation and the use of by-products industrial (corn steep liquor, sugar cane bagasse and molasses) as nutritional source on growth and pigment production by strains MEL 1 and MEL 2 from Aspergillus nidulans. The results showed that, in the presence of 2% corn steep liquor, the cell growth was high while at concentration of 0,2% occurred an increase in the pigment production. When molasses (1 or 10%) was used as carbon sources, the pigment production was inhibited in both concentrations while a high yield of biomass was obtained with 10% molasses. The supplementation of medium with sugar cane bagasse hydrolysate didn´t has a positive effect on pigment production but it provided an increase in the fungal growth. Also was observed that the fungal morphology (pellet size) influenced the production of pigment, being the smaller pellets more suitable for maximum production of the pigment. Thus, the results obtained suggest that corn steep liquor contain substances that stimulate the synthesis of pigment and thus the cultivation with... (Complete abstract click electronic access below)
Due to an increasing interest in the production of actives substances from natural sources, fungi has been considered an important group of microorganisms in function of its enormous potential for exploitation, representing an inexhaustible source to obtain several industrials biotechnology products. Among these products, the melanin pigment produced by fungi Aspergillus nidulans has considerable biotechnological potential to be used in cosmetic formulations, mainly for its antioxidant, anti-inflammatory and photoprotetor activities. However, for a possible practical application of this substance, is necessary to establish the optimal conditions of the fungal culture for large scale production with low cost compared to synthetic melanin. In this context, the objective of this work was to evaluate the influence of volume of culture medium, period of incubation and the use of by-products industrial (corn steep liquor, sugar cane bagasse and molasses) as nutritional source on growth and pigment production by strains MEL 1 and MEL 2 from Aspergillus nidulans. The results showed that, in the presence of 2% corn steep liquor, the cell growth was high while at concentration of 0,2% occurred an increase in the pigment production. When molasses (1 or 10%) was used as carbon sources, the pigment production was inhibited in both concentrations while a high yield of biomass was obtained with 10% molasses. The supplementation of medium with sugar cane bagasse hydrolysate didn´t has a positive effect on pigment production but it provided an increase in the fungal growth. Also was observed that the fungal morphology (pellet size) influenced the production of pigment, being the smaller pellets more suitable for maximum production of the pigment. Thus, the results obtained suggest that corn steep liquor contain substances that stimulate the synthesis of pigment and thus the cultivation with... (Complete abstract click electronic access below)
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Biotecnologia, Resíduos - Agroindústria, Aspergillus nidulans, Biotechnology
Como citar
PRETTI, Taís Simone. Otimização das condições de cultivo do fungo Aspergillus nidulans para produção de melanina usando resíduos agroindustriais. 2009. 73 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Química, 2009.