Diversidade de geofungos em águas do Parque Municipal do Ibirapuera na cidade de São Paulo, SP, Brasil
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Data
2009-10-07
Autores
Takahashi, Juliana Possatto [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
No Córrego do Sapateiro, no Parque Municipal do Ibirapuera, São Paulo, SP, foram coletadas amostras de água (50 mL) até 10 cm de profundidade, em local antes (local 1) e após (local 2) o tratamento da água por flotação, durante época chuvosa (janeiro, fevereiro e março) e época seca (junho, julho e agosto) em 2008. Concomitantemente, foram medidos a temperatura, oxigênio dissolvido, condutividade, e pH da água com equipamento U10 da Horiba. Os coliformes totais e fecais na água foram analisados com Aquatest®. De cada amostra de água, alíquotas de 1 mL foram espalhadas sobre meio de batata-dextrose-ágar, perfazendo 10 placas de Petri para cada local de coleta. Após a incubação durante 10 dias a 22ºC, as colônias foram quantificadas. As mais representativas foram purificadas e identificadas utilizando-se literatura pertinente. Para conduzir testes de patogenicidade foram selecionadas culturas representativas da micota submetendo-as a testes de crescimento a 37ºC, atividade proteásica e fosfolipásica. Os parâmetros abióticos da água não se apresentaram limitantes para a presença de geofungos e foram pouco influenciados pela flotação, exceto o oxigênio dissolvido, que no local 2 apresentou elevações significativas. Nos dois locais o número de coliformes totais e fecais foi predominantemente maior que 8 NMP/dL-1 durante o período de estudo. A quantificação de colônias de fungos variou de 0 a 148 UFC/mL-1. No total foram obtidos 30 táxons de fungos, distribuídos em 141 ocorrências. Vinte táxons foram registrados nos meses quentes e chuvosos e 21 nos demais. Vinte e quatro táxons em 76 ocorrências de fungos foram registrados para o local 1 e 20 táxons em 65 ocorrências no local 2. A micota foi composta por nove gêneros de fungos anamórficos, sendo eles Penicillium sp., Aspergillus sp., Fusarium sp., Cylindrocladium...
In “Córrego do Sapateiro” in the “Parque Municipal do Ibirapuera”, São Paulo city, São Paulo State, Brazil, water samples (50 mL) at 10cm depth were collected in sites before (site 1) and also after (site 2) flotation water treatment, during months in the rainy hot season (January, February and March) and in the dry, cold season (June, July and August) of 2008. Besides, the temperature, dissolved oxygen, conductivity and pH of the water were measured with an U10 Horiba equipment. Total and fecal coliforms were analyzed with Aquatest® kit. From each water sample, aliquots of 1mL were spreaded on potato-dextrose-agar media, totalizing 10 Petri dishes for each collection site. After incubation for 10 days at 22oC, the colonies were quantified. The most representative ones were purified to be identified using current literature. To perform the pathogenicity tests representative strains of the mycota were submitted to growth tests at 37oC, proteolythic and phospholypasic activities. Abiotic parameters of the water were not limitants for the presence of geofungi, and were little influenced by the flotation treatment, except for dissolved oxygen, that presented significant increases at site 2. At the two sites the number of total and fecal coliforms was predominantly higher than 8 NMP/dL-1 during the studied period. The quantity of fungal colonies varied from zero to 148 UFC/mL-1. A total of 30 fungal taxa was obtained, distributed in 141 occurrences. Twenty one taxa were registered in the rainy and hot months, and 21 in the remaining ones. Twenty four taxa, in 76 occurrences were registered for site 1 and 20 taxa in 65 occurrences for site 2. The mycota was composed by nine anamorphic genera, as Penicillium sp., Aspergillus sp., Fusarium sp., Cylindrocladium sp., Pestalotiopsis sp., Trichoderma sp., Cladosporium sp., Geotrichum sp. and Paecilomyces sp., besides two representants... (Complete abstract click electronic access below)
In “Córrego do Sapateiro” in the “Parque Municipal do Ibirapuera”, São Paulo city, São Paulo State, Brazil, water samples (50 mL) at 10cm depth were collected in sites before (site 1) and also after (site 2) flotation water treatment, during months in the rainy hot season (January, February and March) and in the dry, cold season (June, July and August) of 2008. Besides, the temperature, dissolved oxygen, conductivity and pH of the water were measured with an U10 Horiba equipment. Total and fecal coliforms were analyzed with Aquatest® kit. From each water sample, aliquots of 1mL were spreaded on potato-dextrose-agar media, totalizing 10 Petri dishes for each collection site. After incubation for 10 days at 22oC, the colonies were quantified. The most representative ones were purified to be identified using current literature. To perform the pathogenicity tests representative strains of the mycota were submitted to growth tests at 37oC, proteolythic and phospholypasic activities. Abiotic parameters of the water were not limitants for the presence of geofungi, and were little influenced by the flotation treatment, except for dissolved oxygen, that presented significant increases at site 2. At the two sites the number of total and fecal coliforms was predominantly higher than 8 NMP/dL-1 during the studied period. The quantity of fungal colonies varied from zero to 148 UFC/mL-1. A total of 30 fungal taxa was obtained, distributed in 141 occurrences. Twenty one taxa were registered in the rainy and hot months, and 21 in the remaining ones. Twenty four taxa, in 76 occurrences were registered for site 1 and 20 taxa in 65 occurrences for site 2. The mycota was composed by nine anamorphic genera, as Penicillium sp., Aspergillus sp., Fusarium sp., Cylindrocladium sp., Pestalotiopsis sp., Trichoderma sp., Cladosporium sp., Geotrichum sp. and Paecilomyces sp., besides two representants... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Fungos, Flotação, São Paulo (SP), Parque do Ibirapuera (São Paulo, SP), Águas urbanas, Diversidade, Geofungos, Potencial patogênico, Diversity, Flotations, Geofungi, Pathogenic potential, Urban waters
Como citar
TAKAHASHI, Juliana Possatto. Diversidade de geofungos em águas do Parque Municipal do Ibirapuera na cidade de São Paulo, SP, Brasil. 2009. 73 f. Dissertação (mestrado) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro, 2009.