Avaliação clínica, imunológica e da resposta ao tratamento periodontal não-cirúrgico em indivíduos com e sem haplótipos de suscetibilidade genética à periodontite crônica no gene interleucina 8
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Data
2010-03-25
Autores
Corbi, Sâmia Cruz Tfaile [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
A Doença Periodontal (DP) tem caráter multifatorial, com a influência de fatores como a presença de microrganismos periodontopatogênicos, suscetibilidade genética do hospedeiro, reação do sistema imune, hábito de fumar e presença de doenças sistêmicas. O tecido periodontal inflamado produz várias citocinas, dentre elas a interleucina 8 (IL-8). Estudos recentes realizados por este grupo investigaram polimorfismos no gene IL8 em indivíduos com e sem periodontite crônica, onde foram observados indivíduos com 2 vezes mais predisposição genética à DP. O objetivo do presente estudo foi testar a hipótese de que a maior suscetibilidade genética à periodontite crônica dada pelo haplótipo ATC/TTC no gene IL8 seria acompanhada por diferenças nos índices clínicos periodontais, nos níveis da citocina IL-8 no fluido sulcular (FS) e na resposta ao tratamento periodontal não-cirúrgico. Foram selecionados 79 indivíduos divididos quanto à presença ou não do referido haplótipo no gene IL8, de forma que os indivíduos “suscetíveis” e “não suscetíveis” foram subdivididos quanto à presença ou ausência da periodontite crônica. Os indivíduos selecionados foram submetidos a exame clínico periodontal e coletas de FS antes e após 45 dias de finalizado o tratamento periodontal não-cirúrgico. A citocina IL-8 foi quantificada por meio de teste imunoenzimático (ELISA). Como resultado verificou-se que, comparando indivíduos suscetíveis e não-suscetíveis à DP, não houve diferença estatisticamente significante tanto nos índices clínicos periodontais quanto na resposta ao tratamento periodontal realizado, sendo que apenas o volume do FS, no período baseline, apresentou diferença significativa. Assim, a suscetibilidade genética à DP previamente observada nesses indivíduos não...
Periodontal disease is a multifactorial disease which is influenced by factors such as the presence of periodontopathogenic microorganisms, the reaction of the immune system, smoking, genetic susceptibility and the occurrence of systemic diseases. The inflamed periodontal tissue produces several cytokines like Interleukin 8 (IL-8). Recent studies carried out by our research group investigated polymorphisms in the IL8 gene in patients with and without periodontitis, where it was found individuals with a genetic predisposition to periodontitis. The purpose of this study was to test the hypothesis that the genetic susceptibility to chronic periodontitis given by ATC/TTC haplotype in IL8 gene would be linked to differences in the clinical periodontal parameters, IL-8 cytokine levels in the gingival crevicular fluid (GCF) and the response to non-surgical periodontal therapy. Seventy-nine individuals were selected and grouped according to the presence or not of the haplotype given in the IL8 gene, so that the “susceptible” and “nonsusceptible” individuals were subdivided by the presence or absence of chronic periodontitis. The selected individuals were submitted to periodontal clinical exam and the collection of GCF was done before and 45 days after the non-surgical periodontal therapy. The IL-8 cytokine was quantified through an immunoenzymatic assay (ELISA). As a result, it was verified that, comparing susceptible and non-susceptible individuals to chronic periodontitis, there wasn’t any statistically significant difference as in clinical periodontal parameters as in response to non-surgical periodontal therapy. However, the volume of GCF, at baseline, presented a significant difference. Thus, the genetic susceptibility to chronic periodontitis previously observed in these individuals influenced neither... (Complete abstract, click electronic access below)
Periodontal disease is a multifactorial disease which is influenced by factors such as the presence of periodontopathogenic microorganisms, the reaction of the immune system, smoking, genetic susceptibility and the occurrence of systemic diseases. The inflamed periodontal tissue produces several cytokines like Interleukin 8 (IL-8). Recent studies carried out by our research group investigated polymorphisms in the IL8 gene in patients with and without periodontitis, where it was found individuals with a genetic predisposition to periodontitis. The purpose of this study was to test the hypothesis that the genetic susceptibility to chronic periodontitis given by ATC/TTC haplotype in IL8 gene would be linked to differences in the clinical periodontal parameters, IL-8 cytokine levels in the gingival crevicular fluid (GCF) and the response to non-surgical periodontal therapy. Seventy-nine individuals were selected and grouped according to the presence or not of the haplotype given in the IL8 gene, so that the “susceptible” and “nonsusceptible” individuals were subdivided by the presence or absence of chronic periodontitis. The selected individuals were submitted to periodontal clinical exam and the collection of GCF was done before and 45 days after the non-surgical periodontal therapy. The IL-8 cytokine was quantified through an immunoenzymatic assay (ELISA). As a result, it was verified that, comparing susceptible and non-susceptible individuals to chronic periodontitis, there wasn’t any statistically significant difference as in clinical periodontal parameters as in response to non-surgical periodontal therapy. However, the volume of GCF, at baseline, presented a significant difference. Thus, the genetic susceptibility to chronic periodontitis previously observed in these individuals influenced neither... (Complete abstract, click electronic access below)
Descrição
Palavras-chave
Periodontite, Interleucina-8, Tratamento periodontal, Polimorfismo genético, Suscetibilidade à doenças, Interleukin-8, Periodontitis, Polymorphism, genetic, Disease susceptibility
Como citar
CORBI, Sâmia Cruz Tfaile. Avaliação clínica, imunológica e da resposta ao tratamento periodontal não-cirúrgico em indivíduos com e sem haplótipos de suscetibilidade genética à periodontite crônica no gene interleucina 8. 2010. 119 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Odontologia de Araraquara, 2010.