A influência da Diabetes Mellitus no reparo de enxertos ósseos autógenos associados ou não à membrana colágena reabsorvível: estudo histomorfométrico em ratos
Carregando...
Arquivos
Data
2012-07-02
Autores
Meyer, Augusto Cesar de Andrade [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Estadual Paulista (Unesp)
Resumo
O objetivo do presente estudo foi avaliar o processo de reparo ósseo do enxerto ósseo autógeno em bloco, em ratos diabéticos e verificar a influência da membrana colágena reabsorvível sobre este processo. Para tanto, foram utilizados 60 ratos adultos (Rattus norvegicus, variação albinus, Wistar) com sessenta dias de idade, com peso em torno de 200 gramas, que foram divididos em dois grupos experimentais, cada grupo contendo 30 animais: Grupo Teste (D), composto por ratos com Diabetes Mellitus induzida pela administração de aloxana e; Grupo Controle (C) composto por ratos normoglicêmicos. Foram considerados diabéticos os animais que apresentarem glicemia superior a 200 mg/dl. Todos os animais foram submetidos a enxertos na hemi-mandíbula direita e esquerda. Os enxertos na hemi-mandíbula do lado esquerdo foram recobertos por membrana de colágeno reabsorvível, propiciando o recobrimento de todo o bloco enxertado (C-ME e D-ME). Os enxertos realizados na hemi-mandíbula do lado direito foram realizados da mesma maneira, porém sem o recobrimento pela membrana de colágeno (C-E e D-E). A eutanásia dos animais foi realizada por perfusão cardíaca com formalina 4% nos períodos: 0 hora, 7, 14, 21, 45 e 60 dias. As peças foram desmineralizadas com solução de EDTA 10%, pH 7,4, e incluídAS em bloco de parafina e obtenção de cortes seriados com 5 m de espessura. A coloração utilizada foi Hematoxilina e Eosina para análise histológica e histomorfométrica, ambas realizadas em microscopia de luz. As estruturas analisadas foram: matriz óssea do leito receptor e enxerto, matriz óssea neoformada na interface leito receptor e enxerto e ao redor do enxerto, osteócitos, osteoblastos, tecido conjuntivo interposto entre leito receptor e enxerto. A análise histomorfométrica foi realizada...
The aim of this study was to evaluate the bone healing process of autogenous bone graft in diabetic rats and the influence of resorbable collagen membrane. For this purpose, it was 60 adult rats (Rattus norvegicus, Albinus variation, Wistar) sixty days old, weighing around 200 grams, which were divided into two experimental groups, each group containing 30 animals: Test Group (D), rats with diabetes mellitus induced by administration of alloxan and Control Group (C) normoglycemic rats. It was considered diabetic animals showing glucose levels above 200 md/dL. All animals underwent hemi-grafts surgery in the right and left jaws. The hemi-grafts in the left lower jaw were covered by resorbable collagen membrane, allowing the coating of the entire block graft (C-ME and D-ME). The hemi-grafts in the right jaw were performed the same way, but without covering the collagen membrane (C-E and D-E). The animals were euthanasied by cardiac perfusion with 4% formalin after: 0 hour, 7, 14, 21, 45 and 60 days. The pieces were demineralized in 10% EDTA solution, pH 7,4, and each peace was included in paraffin and obtained serial sections with 5 μm thick. The hematoxylin and eosin stain for histological and histomorphometric analysis. The parameters were analyzed: bone matrix graft and recipient bed, newly formed bone matrix at the interface graft and recipient bed and around the graft, osteocytes, osteoblasts, connective tissue interposed between the recipient bed and the graft. Histomorphometric analysis was performed using the computer program NIH Image J 1.35. The data were statistically analyzed using the computer program MINITAB (Minitab, version 14.12, 2004) with analysis of variance (ANOVA) and Tukey´s test, both using the significance of 5%. The results showed that the diabetic rats exhibited at all periods, a greater amount... (Complete abstract click electronic access below)
The aim of this study was to evaluate the bone healing process of autogenous bone graft in diabetic rats and the influence of resorbable collagen membrane. For this purpose, it was 60 adult rats (Rattus norvegicus, Albinus variation, Wistar) sixty days old, weighing around 200 grams, which were divided into two experimental groups, each group containing 30 animals: Test Group (D), rats with diabetes mellitus induced by administration of alloxan and Control Group (C) normoglycemic rats. It was considered diabetic animals showing glucose levels above 200 md/dL. All animals underwent hemi-grafts surgery in the right and left jaws. The hemi-grafts in the left lower jaw were covered by resorbable collagen membrane, allowing the coating of the entire block graft (C-ME and D-ME). The hemi-grafts in the right jaw were performed the same way, but without covering the collagen membrane (C-E and D-E). The animals were euthanasied by cardiac perfusion with 4% formalin after: 0 hour, 7, 14, 21, 45 and 60 days. The pieces were demineralized in 10% EDTA solution, pH 7,4, and each peace was included in paraffin and obtained serial sections with 5 μm thick. The hematoxylin and eosin stain for histological and histomorphometric analysis. The parameters were analyzed: bone matrix graft and recipient bed, newly formed bone matrix at the interface graft and recipient bed and around the graft, osteocytes, osteoblasts, connective tissue interposed between the recipient bed and the graft. Histomorphometric analysis was performed using the computer program NIH Image J 1.35. The data were statistically analyzed using the computer program MINITAB (Minitab, version 14.12, 2004) with analysis of variance (ANOVA) and Tukey´s test, both using the significance of 5%. The results showed that the diabetic rats exhibited at all periods, a greater amount... (Complete abstract click electronic access below)
Descrição
Palavras-chave
Diabetes, Regeneração óssea guiada, Ossos - Enxerto, Implantes dentários
Como citar
MEYER, Augusto Cesar de Andrade. A influência da Diabetes Mellitus no reparo de enxertos ósseos autógenos associados ou não à membrana colágena reabsorvível: estudo histomorfométrico em ratos. 2012. 105 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Odontologia de São José dos Campos, 2012.