Malondialdeído, vitamina E, cortisol, hemograma e enzimas musculares em eqüinos da raça Árabe submetidos ao exercício em esteira de alta
Data
2005
Autores
Silveira, Veridiana Fernandes da [UNESP]
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
O objetivo deste trabalho foi avaliar o estresse oxidativo, em eqüinos, submetidos ao exercício em esteira de alta velocidade. Dez eqüinos adultos da raça Árabe, machos e fêmeas, foram submetidos a um protocolo de exercício de treinamento com velocidades de 1,8m/s por 5 minutos, 4m/s por 3 minutos, 6,2m/s por 2 minutos e 8m/s e 10m/s por 1 minuto e um período de desaquecimento de 2 minutos a 3,0m/s e 1 minuto a 1,6m/s com a esteira em posição horizontal. Foram aferidas a freqüência respiratória, a temperatura retal, a temperatura ambiente e umidade relativa do ar antes e após o exercício e a freqüência cardíaca aferida antes, durante e após o exercício. Os protocolos de exercícios foram realizados seis dias por semana, durante quatro semanas em esteira de alta velocidade, nesta etapa não foram colhidas amostras de sangue dos animais. Após o treinamento os animais foram submetidos ao Teste Padrão de Exercício Progressivo com a esteira inclinada a +6% que consistiu em desafiar os eqüinos com velocidades crescentes até a exaustão. O protocolo do teste foi de 1,8m/s por 5 minutos, 4m/s por 3 minutos, 6m/s por 2 minutos e em seguida fases de 1 minuto com velocidades crescentes onde a maior velocidade atingida pelos animais foi de 11m/s. Foram colhidas amostras de sangue para a dosagem sérica do malondialdeído (MDA), concentração de vitamina E e cortisol, lactato sanguíneo, hemograma, plaquetas, proteína total plasmática, fibrinogênio, atividade enzimática sérica da creatinoquinase (CK), do aspartato aminotransferase (AST) e do lactato desidrogenase (LD) e hemogasometria. A freqüência cardíaca foi acompanhada durante o teste e foram aferidas as temperaturas retais, temperatura ambiente e umidade relativa do ar, antes e após o mesmo. As amostras de sangue foram colhidas antes... .
The objective of this study was to evaluate the oxidative stress in equines submitted to exercise on high-speed treadmill. The animals, 10 adults Arabian horses, males and females, were submitted to training with different speeds of 1.8m/s for 5 minutes, 4m/s for 3 minutes, 6.2m/s for 2 minutes and 8m/s and 10m/s for 1 minute and a subsequent period of chilling of 2 minutes at 3.0m/s and 1 minute at 1.6m/s using the treadmill in horizontal position. Respiratory frequency, rectal and environmental temperature and air relative humidity were taken before and after training. The cardiac frequency was measured before, during and after the exercise. The training protocols were performed six days a week for four weeks on a treadmill; during this period blood samples weren’t collected from the animals. After animal training, Progressive Exercise Pattern Test was performed with the treadmill inclined at +6%, which challenged the equines, increasing speeds until the animals were completely exhausted. The Protocol Test was 1.8m/s for 5 minutes, 4m/s for 3 minutes, 6m/s for 2 minutes and right after, periods of 1 minute with increasing speeds. The horses could reach the maximum speed of 11m/s. Blood samples were taken to evaluate the seric amount of malondialdehyde (MDA), vitamin E and cortisol concentrations, lactate, hemogram, platelets, total plasmatic protein, fibrinogen, seric enzymatic activity of creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD) and blood-gas. The cardiac frequency was measured during the... (Complete abstract, click electronic address below).
The objective of this study was to evaluate the oxidative stress in equines submitted to exercise on high-speed treadmill. The animals, 10 adults Arabian horses, males and females, were submitted to training with different speeds of 1.8m/s for 5 minutes, 4m/s for 3 minutes, 6.2m/s for 2 minutes and 8m/s and 10m/s for 1 minute and a subsequent period of chilling of 2 minutes at 3.0m/s and 1 minute at 1.6m/s using the treadmill in horizontal position. Respiratory frequency, rectal and environmental temperature and air relative humidity were taken before and after training. The cardiac frequency was measured before, during and after the exercise. The training protocols were performed six days a week for four weeks on a treadmill; during this period blood samples weren’t collected from the animals. After animal training, Progressive Exercise Pattern Test was performed with the treadmill inclined at +6%, which challenged the equines, increasing speeds until the animals were completely exhausted. The Protocol Test was 1.8m/s for 5 minutes, 4m/s for 3 minutes, 6m/s for 2 minutes and right after, periods of 1 minute with increasing speeds. The horses could reach the maximum speed of 11m/s. Blood samples were taken to evaluate the seric amount of malondialdehyde (MDA), vitamin E and cortisol concentrations, lactate, hemogram, platelets, total plasmatic protein, fibrinogen, seric enzymatic activity of creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD) and blood-gas. The cardiac frequency was measured during the... (Complete abstract, click electronic address below).
Descrição
Palavras-chave
Equino - Exercícios físicos - Aspectos fisiológicos, Enzimas - Efeito fisiológicos
Como citar
SILVEIRA, Veridiana Fernandes da. Malondialdeído, vitamina E, cortisol, hemograma e enzimas musculares em eqüinos da raça Árabe submetidos ao exercício em esteira de alta. 2005. 92 f. Dissertação (mestrado) - Universidade Estadual Paulista, Faculdade de Medicina Veterinária e Zootecnia de Botucatu, 2005.